Long-linked read sequencing application note

Long-linked Read Sequencing

High-throughput DNA purification for long-read next-generation sequencing

The predominant method for large-scale population genomic analysis relies on the use of short sequencing reads from individual genomes that are aligned on a consensus reference sequence. This approach leads to loss of information about haplotype, genetic phasing, and structural variations. PerkinElmer and 10x Genomics are collaborating to offer efficient automated workflow solutions to provide long-read Next-Generation Sequencing (NGS) technology for DNA extracted from a variety of sample types.

The approach developed by PerkinElmer and 10x Genomics is based on the Chromium™ Genome Solution to exploit the power of Linked-Reads, a unique data type, to tag short reads from the same high molecular weight genomic DNA fragment with molecular barcodes. This unique method allows the placement of short-read information in the context of the whole genome and resolves genetic phasing and structural variation, detecting variants in previously inaccessible and complex regions of the genome. By integrating the Chromium™ Genome Solution to its own DNA extraction chemagic™ instruments, PerkinElmer offers the most efficient approach to the generation of high-throughput NGS results.

PerkinElmer's chemagic™ research instrumentation and kits offer perfect solutions for NGS-grade DNA extraction from many different primary sample types, including saliva, dried blood spots, liquid blood biopsies, and plasma and serum:

  • High DNA yields– e.g, up to 50 µg DNA/ml blood
  • Capacity to handle large volumes of the primary samples – more DNA available for testing
  • Fast processing times– e.g., 96 blood samples in 50 min
  • Tested sample handling technology – lower cross-contamination occurrences
  • Full or partial automated systems – less hands-on time during the nucleic acid purification procedure
  • High levels of DNA integrity – up to 200 kb fragments suitable for long read sequencing
  • Long-term stable nucleic acids – ideal for all genome research applications

For research use only. Not for use in diagnostic procedures.