Streptavidin Magnetic Beads Einleitungstext
Streptavidin M-PVA Magnetic Beads
Magnetic Beads based on Streptavidin (SAV) are commonly used for the isolation and handling of nucleic acids and biotinylated ligands and targets, including antigens and antibodies.
Properties of the M-PVA SAV1/2 Magnetic Beads
- Streptavidin is immobilized covalently on the surface of superparamagnetic polyvinylalcohol beads
- Ready-to-use streptavidin beads for capturing of biotinylated target molecules
- High magnetite content for fast magnetic separation, even from large sample volumes
- Micrometer-sized beads present a high active surface per volume and allow efficient adaptation to specific applications
- Hydrophilic nature of the polyvinylalcohol matrix unspecific binding properties are reduced to a minimum
- Mechanical robustness allows different kinds of processing and facilitates automated use
Specifications of M-PVA SAV Magnetic Beads
Specifications
The beads have a concentration of 25 mg/ml and are stored in PBS buffer, pH 7.2.
The magnetite content is 50-60 %.
Types of M-PVA SAV Magnetic Beads
M-PVA SAV1/2 Magnetic Beads
| Product No. | Name | Specification | Conc. [mg/ml] | Size [µm] | Unit [mg] | Application | Info |
|---|---|---|---|---|---|---|---|
| CMG-227 | M-PVA SAV1 | streptavidin coated | 25 | 0.5 - 1.0 | 50 | separation of biotinylated target molecules | > 50 pmol biotinylated protein, at least 400 pmol biotinylated oligonucleotide or 600 pmol free biotin per 40 μl M-PVA SAV1 respectively |
| CMG-228 | M-PVA SAV2 | streptavidin coated | 25 | 1.0 - 3.0 | 50 | separation of biotinylated target molecules | > 40 pmol biotinylated protein (150 kDa), at least 300 pmol biotinylated oligonucleotide or 520 pmol free biotin per 40 μl M-PVA SAV2 respectively |
Immobilization protocol
Immobilization Protocol
1. Shake bead suspension vigorously and transfer calculated amount.
2. Magnetically separate until the supernatant is clear and wash twice with double volume
of binding buffer. To get optimal separation results particularly for nucleic acid separation
it is recommended to use a binding buffer containing a final salt concentration of at least
0.75 mol/l sodium chloride.
3. Resuspend the prewashed beads with binding buffer to a final concentration of about
4 mg/ml.
4. Add an equal volume of a solution of the biotin-labeled target molecule in binding buffer.
5. Incubate at room temperature using gently rotating or occasional mixing for 15 - 30
minutes.
For complete separation working with very low concentrations of biotinylated substance
the incubation time should be increased to 1 - 2 hours.
6. Wash three times with double volume of binding buffer and resuspend in an appropriate
storage buffer.
Please contact us for further information on our M-PVA SAV1/2 Magnetic Beads.
For research use only. Not for use in diagnostic procedures.