Streptavidin Magnetic Beads Einleitungstext
Streptavidin coated M-PVA Magnetic Beads
The Streptavidin coated M-PVA (M-PVA SAV) Magnetic Beads form a specific class of Revvity’s proprietary M-PVA Magnetic Beads. Detailed characteristics of the M-PVA Magnetic Beads are explained on the overview page. With a magnetite (Fe3O4) content higher than 50% of the total dry weight, encapsulated in a hydrophilic polyvinyl matrix the M-PVA Magnetic Beads separate fast on a magnetic stand or can be easily used in automated systems.
Streptavidin Magnetic Beads
M-PVA Magnetic Beads functionalized with streptavidin are commonly used for the isolation and handling of nucleic acids and biotinylated ligands and targets, including antigens and antibodies. Immobilized Streptavidin on the surface of the beads empower binding and immobilization of biotinylated target molecules, such as oligos, ssDNAs, proteins.
The binding capacity of biotinylated targets in water is generally 500 pmol/mg bead. Higher binding efficiency (800 pmol/mg bead) is achieved in the presence of our coupling buffer.
Interested in receiving M-PVA SAV Magnetic Beads?
Excited about testing our M-PVA SAV Magnetic Beads in your workflow? Then check out the available options on various size ranges and fill out the form below!
Specifications M-PVA SAV Magnetic Beads
Specifications
Binding capacity
- > 3000 pmol free biotin/mg M-PVA SAV Magnetic Beads (Figure 1)
- > 800 pmol ssOligo/mg M-PVA SAV Magnetic Beads (Figure 2)
- > 30 pmol biotinylated protein/mg M-PVA SAV Magnetic Beads
Figure 1. M-PVA SAV1 Magnetic Beads binding capacity of free biotin compared to the competitor streptavidin coated magnetic beads.
Figure 2. Lot-to-lot conformity of M-PVA SAV1 Magnetic Beads by testing the binding capacity of biotinylated ssOligos.
Specifications M-PVA SAV Magnetic Beads
Size
Our M-PVA SAV Magnetic Beads are available in two different size ranges. SAV1 indicates that > 90.0 % of all particles are in the range of 0.60 – 1.25 µm. Whereas > 90.0 % of all SAV2 particles are in the range of 0.80 – 3.0 µm.
Magnetite Content
The high magnetite content of 50 – 60 % (total dry weight) ensures rapid magnetic separation in complex environments even with high volume.
Concentration
The standard concentration is 25 mg/ml, which can be customized upon the requirements of your workflow.
Storage conditions
- Storage buffer: PBS Buffer (pH 7.0), 0.05% NaN3
- Storage temperature: 2 – 8 °C
- Shelf life: 24 months
- Appearance: Brown suspension
Quality
Our M-PVA Magnetic Beads are produced in Germany for over 25 years with reliable quality and constant R&D efforts to support the increasing demands of our customers, and to develop new products to serve the needs of the fast-evolving biotech, pharma, and molecular diagnostics market.
We deliver reproducible binding capacities of > 800 pmol/mg M-PVA Magnetic Bead (M-PVA SAV1 Magnetic Beads, CMG-227) with excellent quality while maintaining an affordable price.
We are ISO 9001 certified supplier.
| Parameter | Acceptance criteria |
|---|---|
| Particle size | M-PVA SAV1 Magnetic Beads > 90.0% of all particles in the range of 0.60 – 1.25 µm M-PVA SAV2 Magnetic Beads > 90.0% of all particles in the range of 0.80 – 3.0 µm |
| Magnetite content | 50 - 60% of total dry weight |
| Sterility | No visible microorganism growth. Bacterial DNA free tested by 16S rDNA PCR |
| Binding properties | > 30 pmol biot. protein per mg |
Workflows with SAV Magnetic Beads
Workflows with SAV M-PVA Magnetic Beads
Effective ribosomal RNA Depletion using Revvity´s Hybridization Technology
The Nextflex® Ribonaut™ rRNA Depletion Kit is used to deplete ribosomal RNA while preserving other RNA species. The subtractive hybridization technology uses biotinylated oligos to selectively capture rRNAs and remove them from the sample using streptavidin coated M-PVA Magnetic Beads.
Product Overview M-PVA SAV Magnetic Beads
M-PVA SAV Magnetic Beads
| Product No. | Name | Functionalization | Size [µm] | Conc. [mg/ml] |
|---|---|---|---|---|
| CMG-227 | M-PVA SAV1 Magnetic Beads | Streptavidin | 0.60 - 1.25 | 25 |
| CMG-228 | M-PVA SAV2 Magnetic Beads | Streptavidin | 0.8 - 3.0 | 25 |
Product Overview M-PVA SAV Magnetic Beads
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For research use only. Not for use in diagnostic procedures.
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