2019-nCoV Coronavirus RNA Purification and Identification Workflow

New 2019-nCoV Coronavirus RNA Purification and Identification Method

Easy and Reliable Isolation of Coronavirus SARS-CoV-2 RNA and Subsequent Analysis

PerkinElmer chemagen has developed a SARS-CoV-2 RNA isolation kit named chemagic Viral DNA/RNA 300 Kit H96 (cat # CMG-1033-S) suitable for the below listed sample materials.

  • Oropharyngeal swab
  • Nasopharyngeal swab
  • Bronchoalveolar lavage (BAL)
  • Sputum
  • Plasma and serum

This kit is for automated isolation from 300 µl of the above sample materials using the chemagic™ 360 instrument (cat. # 2024-0020) equipped with a chemagic 96 Rod Head Set (cat. # CMG-370). The isolation process takes just 60 minutes and the eluted SARS-CoV-2 RNA is ready for subsequent analysis via qPCR, e.g. with real-time PCR, PerkinElmer® SARS-CoV-2 Real-time RT-PCR Assay identifying SARS-CoV-2 ORF1ab and N genes .

SARS-CoV-2 Detection Workflow

Coronavirus Sample Preparation

1. Sample collection with nasal or oropharyngeal swabs. 300 µl BAL, sputum, serum or plasma are common sample materials, too.
2. Nucleic Acid Purification (NAP) using chemagen Technology instruments

SARS-CoV-2 Detection

3a. Reverse Transcription of  RNA to cDNA
3b. qPCR Detection of SARS-CoV-2 specific genes

Kits for SARS-CoV-2 RNA Isolation and qPCR

Catalog no.Kit nameInstrumentInputFormatNo. of preps/kit
Viral DNA/RNA 300 Kit special H96
chemagic™ 360/MSM Iup to 300 µl96 well960
SARS-CoV-2 Real-time RT-PCR AssayPerkinElmer®
SARS-CoV-2 Real-time RT-PCR Assay
third party Real-Time PCR cycler40 µldependent on thermo cycler used48

Instruments Used in the SARS-CoV-2 Detection Workflow

Catalog no.Product nameApplication
2024-0020chemagic™ 360Processing of 96 x 300 µl COVID-19 associated sample materials within 60 minutes
CMG-370chemagic™ 96 Rod Head SetFacilitates processing of 96 samples in parallel on a chemagic™ 360 instrument
variable, please inquireJANUS® G3 workstationFor sample handling and qPCR set up

For research use only. Not for use in diagnostic procedures.