chemagic BBS DNA Kit H96

chemagic BBS DNA Kit H96

IVD-1091 chemagic™ BBS DNA Kit H96

The chemagic BBS DNA Kit H96 is intended for DNA extraction on chemagic instruments from 250 to 400 µl human whole blood, buffy coat, or saliva samples. On the chemagic 360-D instrument, the extraction protocol processes 96 samples in parallel in approximately 79 minutes.

The kit is suitable for diverse IVD downstream applications employing enzymatic amplification and detection of DNA and RNA (e.g., MLPA, PCR, RT-PCR, NGS, methylation assays).

Application data

Extraction performed with the chemagic 360-D instrument on 400 µl 0.9% NaCl solution spiked with 3-15 µg DNA showed good linearity with 72-81% recovery.

Testing with human blood and buffy coat samples showed an average yield of 4.52 pg per WBC or 15.5 µg per 400 µl blood. Expected A260/80 purity ratios of 1.71-1.94 were obtained.

Kit Specifications

Product No.IVD-1091
Preps/Kit960
Sample250 to 400 µl blood, up to 400 µl buffy coat, 400 µl saliva
Instrument(s)chemagic 360-D instrument, other instruments on request
Runtime79 min
Elution volume100 to 300 µl
Required items not supplied in kitchemagic 360-D instrument (prod. no. 2024-0010) with chemagic 360 96 Rod Head Set (prod. no. CMG-370)
pipettes and pipette tips with aerosol barriers
molecular grade water
chemagic Stand 96 (prod. no. CMG-301) (Optional)
isotonic saline solution, sterile (Optional)
ManualsInstructions For Use (IFU) – EN
Instructions For Use (IFU) – EN + DE
Instructions For Use (IFU) – IT
Instructions For Use (IFU) – ES
Instructions For Use (IFU) – FR

Instrument Protocol (ZIP File)
SDSGB
IT
ES
FR

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