New 2019-nCoV Coronavirus RNA Purification and Identification Method
Ultrafast and Reliable Isolation of Coronavirus SARS-CoV-2 RNA and Subsequent Analysis
chemagen has developed a SARS-CoV-2 RNA isolation kit named chemagic Viral DNA/RNA 300 Kit H96 (cat # CMG-1033-S) for automated isolation from 300 µl of below listed sample materials using the chemagic™ 360 instrument (cat. # 2024-0020) equipped with a chemagic 96 Rod Head Set (cat. # CMG-370). The isolation process takes only 18 min with a ultrafast protocol, 31 min with a short protocol and 60 minutes with a regular protocol. The eluted viral RNA is ready for subsequent analysis via RT-PCR.
New ultrafast protocol for viral RNA isolation
Isolate viral SARS-CoV-2 RNA in only 18 min with similar performance in qPCR! Download the ultrafast protocol version and contact us to update your chemagic™ 360 instrument!
For research use only. Not for use in diagnostic procedures.
SARS-CoV-2 Detection Workflow
SARS-CoV-2 Detection Workflow
Coronavirus Sample Preparation
1. Sample collection with nasal or oropharyngeal swabs. 300 µl saliva, sputum, BAL, serum or plasma are common sample materials, too.
2. Nucleic Acid Purification (NAP) using chemagen Technology instruments
Optional: The addition of a homogenization step simplifies the further processing for some sample materials such as saliva. Read more
Detection
3a. Reverse Transcription of RNA to cDNA
3b. RT-PCR Detection of SARS-CoV-2 specific genes
RUO SARS-CoV-2 RNA Isolation
| Catalog No. | Kit Name | Instrument | Input | RUO Sample Type | Format | Preps/kit | Protocols |
|---|---|---|---|---|---|---|---|
| CMG-1033-S | chemagic™ Viral DNA/RNA 300 Kit H96 | chemagic™ 360/MSM I instrument | up to 300 µl | Oropharyngeal swab, nasopharyngeal swab, saliva | 96 well | 960 | Ultrafast - 18 min Short - 31 min Regular - 60 min |
For research use only. Not for use in diagnostic procedures.
CE-IVD Kits for SARS-CoV-2 qPCR
| Catalog No. | Kit Name | Instrument | Input | IVD-CE Sample Type | Detection Of | Preps/kit |
|---|---|---|---|---|---|---|
| SDX-56392 | PKamp™ Respiratory SARS-CoV-2 RT-PCR Panel Assay | Third party Real-Time PCR cycler | 300 µl | Nasopharyngeal swab, oropharyngeal swab | SARS-CoV-2, influenza A, influenza B, respiratory syncytial virus (RSV) | 96 |
| 3501-0010 | PerkinElmer® SARS-CoV-2 RT-qPCR Reagent Kit | Third party Real-Time PCR cycler | 300 µl | Nasopharyngeal swab, oropharyngeal swab | SARS-CoV-2 | 96 |
| MP 2606-0100 | EURORealTime™ SARS-CoV-2* | Third party Real-Time PCR cycler | 300 µl | Throat swabs | SARS-CoV-2 | 100 |
Products may not be licensed in accordance with the laws in all countries, such as the United States and Canada. Please check with your local representative for availability.
The chemagic™ Viral DNA/ RNA 300 Kit H96 (cat. # CMG-1033-S) and the chemagic™ 360 instrument are recommended for the isolation of nucleic acids for use with the PerkinElmer® SARS-CoV-2 Real-time RT-PCR assay or PerkinElmer® SARS-CoV-2 RT-qPCR Reagent Kit.
* The EURORealTime™ SARS-CoV-2 has been validated for CE-IVD registration with the Prepito® Viral DNA/RNA200 Kit (cat. # CMG-2015) with an input volume of 300 µl on the chemagic™ Prepito® instrument.
Find here more products from Euroimmun for Coronavirus Testing.
Detection of COVID-19 Variants of Concern (VOCs)
The observation of SARS-CoV-2 variants necessitates to take a deeper look on positive SARS-CoV-2 results as the observed variants seems to spread faster and/or may cause more severe diseases. Therefore, PerkinElmer provides two solutions for SARS-CoV-2 variant detection:
A NGS solution which can identify all SARS-Cov-2 genetic changes without requiring prior knowledge of variants: NEXTFLEX® Variant-Seq™ SARS-CoV-2 Kit
For research use only. Not for use in diagnostic procedures.
References
Instruments Used in the Coronavirus Detection Workflow
| Catalog No. | Product Name | Application |
|---|---|---|
| 2024-0020 | chemagic™ 360 instrument | Processing of 96 x 300 µl COVID-19 associated sample materials within 60 minutes |
| CMG-370 | chemagic™ 96 Rod Head Set | Facilitates processing of 96 samples in parallel on a chemagic™ 360 instrument |
| variable, please inquire | JANUS® G3 workstation | For sample handling and qPCR set up |
Customer Testimonials
We are using chemagic™ 360 (Automated Nucleic Acid Extraction system) installed in our laboratory for COVID-19 testing. We are running 32 min. short protocol (recently updated by the team) and getting good result with our RT PCR. Due to this short protocol we are able to run approx. 4000 samples / day with two instruments, The instrument supplied has been functioning well, satisfying the requirements of our applications and meeting our expectations for quality and reliability of the data generated.
Department of Microbiology, Government of Rajasthan, S.M.S. Medical School, Jaipur
We had good earlier experience with the chemagic 360 nucleic acid extraction system and found it to be highly versatile, filling many existing and future needs in our operations. When the viral extraction kit and RT-PCR kit became available it was a natural choice to adopt the complete workflow from a single, reliable and local supplier.
Biopsense, Jyväskylä, Finland
Protocol Download
Protocol Download
For research use only. Not for use in diagnostic procedures.
Please note that product labelling (such as kit insert, product label, and kit box) may be different compared to the company branding. Please contact your local representative for further details.