SARS-CoV-2 Testing Workflow

New 2019-nCoV Coronavirus RNA Purification and Identification Method

Easy and Reliable Isolation of Coronavirus SARS-CoV-2 RNA and Subsequent Analysis

PerkinElmer chemagen has developed a SARS-CoV-2 RNA isolation kit named chemagic Viral DNA/RNA 300 Kit H96 (cat # CMG-1033-S) for automated isolation from 300 µl of below listed sample materials using the chemagic™ 360 instrument (cat. # 2024-0020) equipped with a chemagic 96 Rod Head Set (cat. # CMG-370). The isolation process takes just 60 minutes and the eluted viral RNA is ready for subsequent analysis via RT-PCR.

PerkinElmer Coronavirus Testing Workflow

SARS-CoV-2 Detection Workflow

Coronavirus Sample Preparation

1. Sample collection with nasal or oropharyngeal swabs. 300 µl BAL, sputum, serum or plasma are common sample materials, too.
2. Nucleic Acid Purification (NAP) using chemagen Technology instruments


3a. Reverse Transcription of  RNA to cDNA
3b. RT-PCR Detection of SARS-CoV-2 specific genes

RUO Kits for RNA Isolation and qPCR

Catalog no.Kit nameInstrumentInputRUO sample typeFormatNo. of preps/kit
Viral DNA/RNA 300 Kit H96
chemagic™ 360/MSM I instrumentup to 300 µlOropharyngeal swab, Nasopharyngeal swab96 well960
SARS-CoV-2-PCR-RUOPerkinElmer® SARS-CoV-2 Nucleic Acid Kit (RUO)third party Real-Time PCR cycler400 µlOropharyngeal swab, Nasopharyngeal swabdependent on thermo cycler used48

For research use only. Not for use in diagnostic procedures.

CE-IVD Kit for SARS-CoV-2 qPCR

Catalog no.Kit nameInstrumentInputIVD-CE sample typeFormatNo. of preps/kit
Covid-19-PCR-AUS-CPerkinElmer® SARS-CoV-2 Real-time RT-PCR Assaythird party Real-Time PCR cycler400 µlNasopharyngeal swab, Oropharyngeal swabdependent on thermo cycler used48
3501-0010PerkinElmer® SARS-CoV-2 RT-qPCR Reagent Kitthird party Real-Time PCR cycler300 µlNasopharyngeal swab, Oropharyngeal swabdependent on thermo cycler used96
MP 2606-0100EURORealTime™ SARS-CoV-2*third party Real-Time PCR cycler300 µlThroat swabsdependent on thermo cycler used100

The chemagic™ Viral DNA/ RNA 300 Kit H96 (cat. # CMG-1033-S) and the chemagic™ 360 instrument are recommended for the isolation of nucleic acids for use with the PerkinElmer® SARS-CoV-2 Real-time RT-PCR assay or PerkinElmer® SARS-CoV-2 RT-qPCR Reagent Kit.

* The EURORealTime™ SARS-CoV-2 has been validated for CE-IVD registration with the Prepito® Viral DNA/RNA200 Kit (cat. # CMG-2015) with an input volume of 300 µl on the chemagic™ Prepito® instrument.

Find here more products from Euroimmun for Coronavirus Testing.


MacKay, M.J., Hooker, A.C., Afshinnekoo, E. et al. The COVID-19 XPRIZE and the need for scalable, fast, and widespread testing. Nat Biotechnol (2020).

Instruments Used in the Coronavirus Detection Workflow

Catalog no.Product nameApplication
2024-0020chemagic™ 360 instrumentProcessing of 96 x 300 µl COVID-19 associated sample materials within 60 minutes
CMG-370chemagic™ 96 Rod Head SetFacilitates processing of 96 samples in parallel on a chemagic™ 360 instrument
variable, please inquire
JANUS® G3 workstation
For sample handling and qPCR set up

Protocol Download

Protocol Download

For research use only. Not for use in diagnostic procedures.