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SARS-CoV-2 Testing Workflow

New 2019-nCoV Coronavirus RNA Purification and Identification Method

Ultrafast and Reliable Isolation of Coronavirus SARS-CoV-2 RNA and Subsequent Analysis

chemagen has developed a SARS-CoV-2 RNA isolation kit named chemagic Viral DNA/RNA 300 Kit H96 (cat # CMG-1033-S) for automated isolation from 300 µl of below listed sample materials using the chemagic™ 360 instrument (cat. # 2024-0020) equipped with a chemagic 96 Rod Head Set (cat. # CMG-370). The isolation process takes only 18 min with a ultrafast protocol, 31 min with a short protocol and 60 minutes with a regular protocol. The eluted viral RNA is ready for subsequent analysis via RT-PCR.

Coronavirus PCR testing workflow

Ultrafast protocol for viral RNA isolation

Isolate viral SARS-CoV-2 RNA in only 18 min with similar performance in qPCR! Download the ultrafast protocol version and contact us to update your chemagic™ 360 instrument!

For research use only. Not for use in diagnostic procedures.

SARS-CoV-2 Detection Workflow

SARS-CoV-2 Detection Workflow

Coronavirus Sample Preparation

1. Sample collection with nasal or oropharyngeal swabs. 300 µl saliva, sputum, BAL, serum or plasma are common sample materials, too.
2. Nucleic Acid Purification (NAP) using chemagen Technology instruments

Optional: The addition of a homogenization step simplifies the further processing for some sample materials such as saliva. 


3a. Reverse Transcription of  RNA to cDNA
3b. RT-PCR Detection of SARS-CoV-2 specific genes

RUO SARS-CoV-2 RNA Isolation

Catalog No.Kit NameInstrumentInputRUO Sample TypeFormatPreps/kitProtocols
Viral DNA/RNA 300 Kit H96
chemagic™ 360/MSM I
up to 300 µlOropharyngeal swab,
swab, saliva
96 well960Ultrafast - 18 min
Short - 31 min
Regular - 60 min

For research use only. Not for use in diagnostic procedures.

CE-IVD Kits for SARS-CoV-2 qPCR

Catalog No.Kit NameInstrumentInputIVD-CE Sample TypeDetection OfPreps/kit
3504-0010PKamp™ Respiratory SARS-CoV-2 RT-PCR Panel AssayThird party Real-Time PCR cycler300 µlNasopharyngeal swab, oropharyngeal swabSARS-CoV-2, influenza A, influenza B, respiratory syncytial virus (RSV)96
MP 2606-0100EURORealTime™ SARS-CoV-2*Third party Real-Time PCR cycler300 µlThroat swabsSARS-CoV-2100

Products may not be licensed in accordance with the laws in all countries, such as the United States and Canada. Please check with your local representative for availability.

The chemagic™ Viral DNA/ RNA 300 Kit H96 (cat. # CMG-1033-S) and the chemagic™ 360 instrument are recommended for the isolation of nucleic acids for use with the Revvity SARS-CoV-2 Real-time RT-PCR assay or Revvity SARS-CoV-2 RT-qPCR Reagent Kit.
* The EURORealTime™ SARS-CoV-2 has been validated for CE-IVD registration with the Prepito® Viral DNA/RNA200 Kit (cat. # CMG-2015) with an input volume of 300 µl on the chemagic™ Prepito® instrument.
Find here more products from Euroimmun for Coronavirus Testing.

Instruments Used in the Coronavirus Detection Workflow

Catalog No.Product NameApplication
2024-0020chemagic™ 360 instrumentProcessing of 96 x 300 µl COVID-19 associated sample materials within 60 minutes
CMG-370chemagic™ 96 Rod Head SetFacilitates processing of 96 samples in parallel on a chemagic™ 360 instrument
variable, please inquire
JANUS® G3 workstationFor sample handling and qPCR set up

Customer Testimonials

We are using chemagic™ 360 (Automated Nucleic Acid Extraction system) installed in our laboratory for COVID-19 testing. We are running 32 min. short protocol (recently updated by the team) and getting good result with our RT PCR. Due to this short protocol we are able to run approx. 4000 samples / day with two instruments, The instrument supplied has been functioning well, satisfying the requirements of our applications and meeting our expectations for quality and reliability of the data generated.

Department of Microbiology, Government of Rajasthan, S.M.S. Medical School, Jaipur

We had good earlier experience with the chemagic 360 nucleic acid extraction system and found it to be highly versatile, filling many existing and future needs in our operations. When the viral extraction kit and RT-PCR kit became available it was a natural choice to adopt the complete workflow from a single, reliable and local supplier.

Biopsense, Jyväskylä, Finland

Protocol Download

Protocol Download

For research use only. Not for use in diagnostic procedures.