New 2019-nCoV Coronavirus RNA Purification and Identification Method
Fast, Easy and Reliable Isolation of Coronavirus SARS-CoV-2 RNA and Subsequent Analysis
PerkinElmer chemagen has developed a SARS-CoV-2 RNA isolation kit named chemagic Viral DNA/RNA 300 Kit H96 (cat # CMG-1033-S) for automated isolation from 300 µl of below listed sample materials using the chemagic™ 360 instrument (cat. # 2024-0020) equipped with a chemagic 96 Rod Head Set (cat. # CMG-370). The isolation process takes just 60 minutes and only 31 minutes with an optimized short protocol. The eluted viral RNA is ready for subsequent analysis via RT-PCR.
SARS-CoV-2 Detection Workflow
Coronavirus Sample Preparation
1. Sample collection with nasal or oropharyngeal swabs. 300 µl saliva, sputum, BAL, serum or plasma are common sample materials, too.
2. Nucleic Acid Purification (NAP) using chemagen Technology instruments
Detection
3a. Reverse Transcription of RNA to cDNA
3b. RT-PCR Detection of SARS-CoV-2 specific genes
RUO Kits for RNA Isolation and qPCR
| Catalog no. | Kit name | Instrument | Input | RUO sample type | Detection of | Format | No. of preps/kit |
|---|---|---|---|---|---|---|---|
| CMG-1033-S | chemagic™ Viral DNA/RNA 300 Kit H96 | chemagic™ 360/MSM I instrument | up to 300 µl | Oropharyngeal swab, nasopharyngeal swab, saliva | n.a. | 96 well | 960 |
| SARS-CoV-2-PCR-RUO | PerkinElmer® SARS-CoV-2 Nucleic Acid Kit (RUO) | Third party Real-Time PCR cycler | 400 µl | Oropharyngeal swab, nasopharyngeal swab | SARS-CoV-2 | dependent on thermo cycler used | 48 |
For research use only. Not for use in diagnostic procedures.
FDA EUA authorized Kit for qPCR
| Catalog no. | Kit name | Instrument | Input | FDA EUA sample type | Detection of | Pooling | No. of preps/kit |
|---|---|---|---|---|---|---|---|
| 2019-nCoV-PCR-AUS | PerkinElmer® New Coronavirus Nucleic Acid Detection Kit | Third party Real-Time PCR cycler | 300 µl | Oropharyngeal swab, nasopharyngeal swab, anterior nasal swab | SARS-CoV-2 | Yes, up to 5 individuals | 48 |
For emergency use authorization only. Products may not be licensed in accordance with the laws in all countries. Please check with your local representative for availability.
CE-IVD Kits for SARS-CoV-2 qPCR
| Catalog no. | Kit name | Instrument | Input | IVD-CE sample type | Detection of | Pooling | No. of preps/kit |
|---|---|---|---|---|---|---|---|
| SDX-56392 | PKamp™ Respiratory SARS-CoV-2 RT-PCR Panel Assay | Third party Real-Time PCR cycler | 300 µl | Nasopharyngeal swab, oropharyngeal swab | SARS-CoV-2, influenza A, influenza B, respiratory syncytial virus (RSV) | No | 96 |
| Covid-19-PCR-AUS-C | PerkinElmer® SARS-CoV-2 Real-time RT-PCR Assay | Third party Real-Time PCR cycler | 400 µl | Nasopharyngeal swab, oropharyngeal swab, Saliva | SARS-CoV-2 | Yes, up to 5 individuals | 48 |
| 3501-0010 | PerkinElmer® SARS-CoV-2 RT-qPCR Reagent Kit | Third party Real-Time PCR cycler | 300 µl | Nasopharyngeal swab, oropharyngeal swab | SARS-CoV-2 | No | 96 |
| MP 2606-0100 | EURORealTime™ SARS-CoV-2* | Third party Real-Time PCR cycler | 300 µl | Throat swabs | SARS-CoV-2 | No | 100 |
Products may not be licensed in accordance with the laws in all countries, such as the United States and Canada. Please check with your local representative for availability.
The chemagic™ Viral DNA/ RNA 300 Kit H96 (cat. # CMG-1033-S) and the chemagic™ 360 instrument are recommended for the isolation of nucleic acids for use with the PerkinElmer® SARS-CoV-2 Real-time RT-PCR assay or PerkinElmer® SARS-CoV-2 RT-qPCR Reagent Kit.
* The EURORealTime™ SARS-CoV-2 has been validated for CE-IVD registration with the Prepito® Viral DNA/RNA200 Kit (cat. # CMG-2015) with an input volume of 300 µl on the chemagic™ Prepito® instrument.
Find here more products from Euroimmun for Coronavirus Testing.
Instruments Used in the Coronavirus Detection Workflow
| Catalog no. | Product name | Application |
|---|---|---|
| 2024-0020 | chemagic™ 360 instrument | Processing of 96 x 300 µl COVID-19 associated sample materials within 60 minutes |
| CMG-370 | chemagic™ 96 Rod Head Set | Facilitates processing of 96 samples in parallel on a chemagic™ 360 instrument |
| variable, please inquire | JANUS® G3 workstation | For sample handling and qPCR set up |
Customer Testimonial
We are using PerkinElmer model chemagic™ 360 (Automated Nucleic Acid Extraction system) installed in our laboratory for COVID-19 testing. We are running 32 min. short protocol (recently updated by the PerkinElmer Team) and getting good result with our RT PCR. Due to this short protocol we are able to run approx. 4000 samples / day with two instruments, The instrument supplied has been functioning well, satisfying the requirements of our applications and meeting our expectations for quality and reliability of the data generated.
Department of Microbiology, Government of Rajasthan, S.M.S. Medical School, Jaipur
Protocol Download
Protocol Download
For research use only. Not for use in diagnostic procedures.