M-PVA Magnetic Beads


Magnetic Beads Based on Polyvinyl Alcohol

chemagen started its business with the development, functionalization, and production of magnetic particles based on polyvinyl alcohol, referred to as M-PVA Magnetic Beads. These beads represent a new class of particles and allow applications in nearly every scientific field requiring the isolation of specific biomolecules.

Develop your own assay with our M-PVA Magnetic Beads. Contact our OEM team for further support.

The picture shows a schematic representation of the M-PVA Magnetic Bead structure. Particles of magnetite are embedded in the polyvinyl alcohol matrix conferring paramagnetic properties to the beads.

The surface of the M-PVA Magnetic Beads can be functionalized with many different groups and modified with individually tuneable loadings. The figure on the left shows a selection of functionalized, activated beads offered by chemagen as standard and ready-to-use reagents.

Streptavidin coated M-PVA Magnetic Beads

Applications of M-PVA Magnetic Beads

Thanks to their hydrophilic surface, M-PVA Magnetic Beads show a very little affinity for most biological molecules, opposite to the majority of commercially available beads that are based on hydrophobic materials such as polystyrene. This feature makes activated M-PVA Magnetic Beads ideal for the separation of specific molecules from complex mixtures.

Standard functionalized M-PVA Magnetic Beads:

  • Carboxylated beads
  • Oligo(dT)
  • Streptavidin (SAV)
  • N-hydroxysuccinimidyl (NHS) ester
  • Primary amine functionalized
  • Epoxide functionalized
  • OH-functionalized beads

Major applications:

  • Isolation of nucleic acids
  • Detection or isolation of specific sequences or species of nucleic acids
  • Preparation of molecule libraries (e.g. oligonucleotides, DNA, RNA, proteins, or smaller molecules) using combinatorial strategies
  • Screening and selection of DNA libraries
  • Binding of biotinylated proteins
  • Affinity purification of proteins (e.g. antibodies or lectins)
M-PVA Magnetic Beads

Outstanding properties

  • High magnetite content for fast magnetic separation, even from large sample volumes
  • Polydisperse particles for a uniform reproducibility of magnetic separation
  • µm sized beads present a high active surface per volume and allow efficient adaptation to specific applications
  • Mechanical robustness allows different kinds of processing and facilitates automated use
  • Chemical functionalization of the matrix with different modifications for high and specific binding capacity
  • Hydrophilicity of the PVA surface, leading to low non-specific protein binding
  • High thermal stability enabling easy sterilization by autoclaving

Types of M-PVA Magnetic Beads

M-PVA Magnetic Beads

Product No.Name SpecificationConc.
CMG-200M-PVA 011hydroxylated500.5 - 1.0500various binding capacitystored in H2O at room temperature
CMG-201M-PVA 012hydroxylated501.0 - 3.0500various binding capacitystored in H2O at room temperature
CMG-206M-PVA C21 highly carboxylated500.5 - 1.0500coupling of proteins, nucleic acids and other ligands with amino functionalities> 950 µmol COOH/g
CMG-207M-PVA C22highly carboxylated501.0 - 3.0500coupling of proteins, nucleic acids and other ligands with amino functionalities> 950 µmol COOH/g
CMG-216M-PVA E01epoxidated 500.5 - 1.0500binding capacity for amino and thiol groups in coupling buffer with pH >8~ 200 µmol/g
CMG-221M-PVA Ak11NHS activated250.5 - 1.050binding of proteins or ligands with amino functionalities> 650 µmol NHS/g
CMG-222M-PVA Ak12NHS activated251.0 - 3.050binding of proteins or ligands with amino functionalities> 550 µmol NHS/g
CMG-227M-PVA SAV1streptavidin coated250.5 - 1.050separation
of biotinylated target molecules
> 50 pmol biotinylated protein, at least 400 pmol biotinylated oligonucleotide or 600 pmol free biotin per 40 μl M-PVA SAV1 respectively
CMG-228M-PVA SAV2streptavidin coated251.0 - 3.050separation
of biotinylated target molecules
> 40 pmol biotinylated protein (150 kDa), at least 300 pmol biotinylated oligonucleotide or 520 pmol free biotin per 40 μl M-PVA SAV2 respectively
CMG-231M-PVA OdT2oligo(dT) coated251.0 - 3.050coupling of poly-adenylated nucleic acids> 45 pmol dA30
CMG-237M-PVA Ak11NHS activated250.5 - 1.0500binding of proteins or ligands with amino functionalities> 650 µmol NHS/g
CMG-238M-PVA Ak12NHS activated251.0 - 3.0500binding of proteins or ligands with amino functionalities> 550 µmol NHS/g

If you are looking for magnetic particles for an application or with a specific functionalization not listed above, please contact us.

For research use only. Not for use in diagnostic procedures.