Einleitungstext
Magnetic Beads Based on Polyvinyl Alcohol
chemagen started its business with the development, functionalization, and production of magnetic particles based on polyvinyl alcohol, referred to as M-PVA Magnetic Beads. These beads represent a new class of particles and allow applications in nearly every scientific field requiring the isolation of specific biomolecules.
Develop your own assay with our M-PVA Magnetic Beads. Contact our OEM team for further support.
The picture shows a schematic representation of the M-PVA Magnetic Bead structure. Particles of magnetite are embedded in the polyvinyl alcohol matrix conferring paramagnetic properties to the beads.
The surface of the M-PVA Magnetic Beads can be functionalized with many different groups and modified with individually tuneable loadings. The figure on the left shows a selection of functionalized, activated beads offered by chemagen as standard and ready-to-use reagents.
Applications of M-PVA Magnetic Beads
Thanks to their hydrophilic surface, M-PVA Magnetic Beads show a very little affinity for most biological molecules, opposite to the majority of commercially available beads that are based on hydrophobic materials such as polystyrene. This feature makes activated M-PVA Magnetic Beads ideal for the separation of specific molecules from complex mixtures.
Standard functionalized M-PVA Magnetic Beads:
- Carboxylated beads
- Oligo(dT)
- Streptavidin (SAV)
- N-hydroxysuccinimidyl (NHS) ester
- Primary amine functionalized
- Epoxide functionalized
- OH-functionalized beads
Major applications:
- Isolation of nucleic acids
- Detection or isolation of specific sequences or species of nucleic acids
- Preparation of molecule libraries (e.g. oligonucleotides, DNA, RNA, proteins, or smaller molecules) using combinatorial strategies
- Screening and selection of DNA libraries
- Binding of biotinylated proteins
- Affinity purification of proteins (e.g. antibodies or lectins)
Outstanding properties
- High magnetite content for fast magnetic separation, even from large sample volumes
- Polydisperse particles for a uniform reproducibility of magnetic separation
- µm sized beads present a high active surface per volume and allow efficient adaptation to specific applications
- Mechanical robustness allows different kinds of processing and facilitates automated use
- Chemical functionalization of the matrix with different modifications for high and specific binding capacity
- Hydrophilicity of the PVA surface, leading to low non-specific protein binding
- High thermal stability enabling easy sterilization by autoclaving
Types of M-PVA Magnetic Beads
M-PVA Magnetic Beads
| Product No. | Name | Specification | Conc. [mg/ml] | Size [µm] | Unit [mg] | Application | Info |
|---|---|---|---|---|---|---|---|
| CMG-200 | M-PVA 011 | hydroxylated | 50 | 0.5 - 1.0 | 500 | various binding capacity | stored in H2O at room temperature |
| CMG-201 | M-PVA 012 | hydroxylated | 50 | 1.0 - 3.0 | 500 | various binding capacity | stored in H2O at room temperature |
| CMG-206 | M-PVA C21 | highly carboxylated | 50 | 0.5 - 1.0 | 500 | coupling of proteins, nucleic acids and other ligands with amino functionalities | > 950 µmol COOH/g |
| CMG-207 | M-PVA C22 | highly carboxylated | 50 | 1.0 - 3.0 | 500 | coupling of proteins, nucleic acids and other ligands with amino functionalities | > 950 µmol COOH/g |
| CMG-216 | M-PVA E02 | epoxidated | 50 | 1.0 - 3.0 | 500 | binding capacity for amino and thiol groups in coupling buffer with pH >8 | ~ 200 µmol/g |
| CMG-221 | M-PVA Ak11 | NHS activated | 25 | 0.5 - 1.0 | 50 | binding of proteins or ligands with amino functionalities | > 650 µmol NHS/g |
| CMG-222 | M-PVA Ak12 | NHS activated | 25 | 1.0 - 3.0 | 50 | binding of proteins or ligands with amino functionalities | > 550 µmol NHS/g |
| CMG-227 | M-PVA SAV1 | streptavidin coated | 25 | 0.5 - 1.0 | 50 | separation of biotinylated target molecules | > 50 pmol biotinylated protein, at least 400 pmol biotinylated oligonucleotide or 600 pmol free biotin per 40 μl M-PVA SAV1 respectively |
| CMG-228 | M-PVA SAV2 | streptavidin coated | 25 | 1.0 - 3.0 | 50 | separation of biotinylated target molecules | > 40 pmol biotinylated protein (150 kDa), at least 300 pmol biotinylated oligonucleotide or 520 pmol free biotin per 40 μl M-PVA SAV2 respectively |
| CMG-231 | M-PVA OdT2 | oligo(dT) coated | 25 | 1.0 - 3.0 | 50 | coupling of poly-adenylated nucleic acids | > 45 pmol dA30 |
| CMG-237 | M-PVA Ak11 | NHS activated | 25 | 0.5 - 1.0 | 500 | binding of proteins or ligands with amino functionalities | > 650 µmol NHS/g |
| CMG-238 | M-PVA Ak12 | NHS activated | 25 | 1.0 - 3.0 | 500 | binding of proteins or ligands with amino functionalities | > 550 µmol NHS/g |
If you are looking for magnetic particles for an application or with a specific functionalization not listed above, please contact us.
For research use only. Not for use in diagnostic procedures.