DNA and RNA from saliva

Saliva has become an important source of nucleic acid material for human genetic testing. It is non-invasive, which makes it a preferred choice for individuals who dislike needles. The Revvity chemagen solutions provide high yield and purity genomic DNA and total RNA from a minimal amount of sample. We observe high molecular weight gDNA and high integrity total RNA and they work with several downstream applications including sequencing (Next-Generation, Whole Genome, Whole Exome, Sanger, and long-read), quantitative PCR, and more.

References

1. Manzoor, M., Lommi, S., Furuholm, J. et al. High abundance of sugar metabolisers in saliva of children with caries. Sci Rep 11,4424 (2021). 
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3. Yao RA, Akinrinade O, Chaix M, Mital S. Quality of whole genome sequencing from blood versus saliva derived DNA in cardiac patients. BMC Med Genomics. (2020)
4. Senok, A., Alsuwaidi, H., Atrah, Y., Al Ayedi, O., Al Zahid, J., Han, A., Al Marzooqi, A., Al Heialy, S., Altrabulsi, B., AbdelWareth, L., Idaghdour, Y., Ali, R., Loney, T., & Alsheikh-Ali, A. . Saliva as an Alternative Specimen for Molecular COVID-19 Testing in Community Settings and Population-Based Screening. Infection and drug resistance, 13, 3393–3399. (2020)