A robust and high yield DNA extraction protocol from bovine liver utilizing the OMNI Bead Ruptor Elite™ Bead Mill Homogenizer in combination with automated extraction on the chemagic™ 360 Nucleic Acid Extractor

The processing of human and animal tissue samples for molecular analysis has become a cornerstone of research efforts across a multitude of disciplines. In particular, the processing of soft tissues, such as liver samples, is proving critical for everything from drug development research to anatomy research examining embryology and stem cell development. Nucleic acid extraction is one of the commonly performed processes employed when preparing these samples for downstream analysis, allowing researchers to produce high qualities and quantities of DNA and/or RNA from their samples.

In the following application note,  we demonstrate the utility of OMNI Bead Ruptor Elite™ Bead Mill Homogenizer in combination with the chemagic™ 360 instrument for a robustly reliable workflow resulting in high qualities and quantities of DNA from bovine liver samples. This protocol prescribes a complete workflow from sample lysis through automated DNA extraction with a focus on complete sample homogenization and reliability in comparison to enzymatic lysis.