The chemagic Prime instrument for fully automated nucleic acid purification
The chemagic™ Prime™ instrument for flexible automated DNA and RNA workflows
The chemagic Prime instrument is a powerful solution for high-throughput automated DNA and RNA purification and automated assay setup. The chemagic Prime instrument combines chemagen’s patented M-PVA Magnetic Bead Technology with liquid handling capabilities, providing automated sample preparation by handling various primary sample tubes and fully automated isolation of ultra-pure, high integrity nucleic acids from diverse sample types. The instrument performs downstream processing e.g. PCR setup, normalization or eluate transfer to storage tubes.
The platform boasts fully traceable sample preparation, consistent performance and the flexibility to meet custom workflows demands. Benefit from its diverse tube handling, automated barcode tracking, quality assurance features to reduce cross-contamination, high throughput capability (up to 192 samples/run), large sample volume range (up to 10 ml), and user-friendly operation. With a wide selection of kits, the instrument can isolate DNA, RNA, cfDNA, pathogen nucleic acids and more, enabling the usage of one system for multiple assays. The technology behind it utilizes transiently magnetized rods to attract beads after binding to nucleic acids, obtaining nucleic acids of high yield, integrity and purity, proven to increase downstream success in diverse applications including long read sequencing, NGS, MLPA, PCR and more.
Features
Key Features
- Performance - High yields, integrity and purity of nucleic acids with proven performance in long-read sequencing, NGS, PCR, MLPA etc.
- Flexibility - sample volumes up to 10 ml, 1- 192 samples per run, diverse tubes/sample types in a single run, downstream normalization/assay setup/eluate transfer, customized pipetting for viscous samples
- Automated traceability – On-deck 1D/2D barcode scanning, LIMS compatible log files, customizable run reports
- Quality assurance – 100% traceability, tracked reagent/tip consumption, safe travel paths, UV disinfection, drop cover
- Efficiency - Optimized reagent dispensing (no expensive cartridges), multi-kit use with switchbox, on-deck heating/cooling/shaking options
- Ease of operation – status lights, fast start-up, intuitive software, guided setup and maintenance, user-friendly programming, email notification of errors
Features
Applications with chemagic Prime instrument
High-throughput blood donor screening
High-throughput blood donor screening
Using the chemagic Prime instrument, up to 552 blood bags are analyzed routinely, with high traceability and sensitivity for detecting HIV (AIDS virus), HCV (hepatitis C virus), HBV (hepatitis B virus) and malaria with the NAT Plus HIV/HBV/HCV/Malaria Kit developed by the Oswaldo Cruz Foundation (Bio-Manguinhos/Fiocruz). This has helped reduce the waiting period to donate blood after visiting an endemic area from 1 year to 1 month and has successfully reduced the risk of pathogen transmission due to contaminated blood1.
Genetic study of Autism Spectrum Disorder in Vietnamese children
The chemagic Prime instrument played a crucial role in isolating high-quality DNA from 254 saliva samples, which was essential for the study’s subsequent high resolution SNP genotyping. This technique enabled the identification of pathogenic and likely pathogenic mutations in children with autism spectrum disorder. The automation and precision of the chemagic Prime system significantly enhanced the efficiency and consistency of sample processing, underscoring its importance in large-scale genetic research2.
Pathogen surveillance from swabs and wastewater
Multiple studies have demonstrated the application of the chemagic Prime instrument for the high-throughput and sensitive detection of SARS-CoV-2 from samples including blood, pharyngeal swabs, environmental surface swabs and wastewater3,4,5,6. The ability of chemagic Kits to extract nucleic acids from a wide range of pathogens and the unique feature of the chemagic Prime instrument in handling diverse sample/eluate tubes and plates makes it a reliable yet versatile solution.
chemagic Prime Kits
Kits are available for the isolation of DNA and RNA from various specimens such as whole blood, saliva, plasma, and many more. We provide chemagic Prime Kits that have been validated on the chemagic Prime instrument.
All kits come ready-to-use with plastic consumables included and are based on well-established chemagic M-PVA Magnetic Bead and buffer chemistry. For applications not listed, please contact us.
References
- Costa E, Rocha D, Lopes JIF, et al. Detection of Plasmodium spp. in asymptomatic blood donors by the new Brazilian NAT PLUS HIV/HBV/HCV/Malaria Bio-Manguinhos kit. Transfusion. 2024;64(3):501-509. doi:10.1111/trf.17726
- Bui DT, Ton ANV, Nguyen CTD, et al. Pathogenic/likely pathogenic mutations identified in Vietnamese children diagnosed with autism spectrum disorder using high-resolution SNP genotyping platform. Sci Rep. 2024;14(1):2360. Published 2024 Jan 29. doi:10.1038/s41598-024-52777-y
- Döhla M, Schulte B, Wilbring G, et al. SARS-CoV-2 in Environmental Samples of Quarantined Households. Viruses. 2022;14(5):1075. Published 2022 May 17. doi:10.3390/v14051075
- Lou EG, Sapoval N, McCall C, et al. Direct comparison of RT-ddPCR and targeted amplicon sequencing for SARS-CoV-2 mutation monitoring in wastewater. Sci Total Environ. 2022;833:155059. doi:10.1016/j.scitotenv.2022.155059
- Streeck H, Schulte B, Kümmerer BM, et al. Infection fatality rate of SARS-CoV2 in a super-spreading event in Germany. Nat Commun. 2020;11(1):5829. Published 2020 Nov 17. doi:10.1038/s41467-020-19509-y
- LaTurner ZW, Zong DM, Kalvapalle P, et al. Evaluating recovery, cost, and throughput of different concentration methods for SARS-CoV-2 wastewater-based epidemiology. Water Res. 2021;197:117043. doi:10.1016/j.watres.2021.117043
For research use only. Not for use in diagnostic procedures.