Automated DNA Isolation and Library Preparation
Automated DNA purification from FFPE samples for NGS library preparation
Next-generation sequencing (NGS) has revolutionized the field of genomic research. The dropping costs and technology improvements have made feasible the application of NGS to high-throughput studies, providing a new and powerful tool to scientists working in fields as diverse as plant biology and human genetics. A crucial step in the NGS workflow is the preparation of the library for sequencing. A poor-quality library can lead to profound technical artifacts that might influence the results and, ultimately, data interpretation. The isolation of pure high molecular weight DNA or full-length RNA and a consistent and robust fragmentation protocol are the foundations to successful library preparation. To ensure that both conditions are met, chemagen has integrated the Covaris® Focused-ultrasonicator to its nucleic acid purification workflow. By exploiting the chemagic™ technology, ensures optimal extraction of DNA and RNA from a wide range of sample types. The purified nucleic acid is then fragmented using the Covaris® Focused-ultrasonicator. The integration of the Covaris® system with the chemagic™ workflows ensures unparalleled high-throughput capabilities from primary sample to DNA and RNA fragments of the desired size ready for library preparation.
- High DNA yields
- Fast processing times
- Tested sample handling technology – lower cross-contamination occurrences
- Full or partial automated systems – less hands-on time during the nucleic acid purification procedure
- High levels of DNA integrity – up to 200 kb fragments suitable for the most complex genomic analyses
- Long-term stable nucleic acids – ideal for biobanking and clinical research applications
For more information, please download the application note.
For research use only. Not for use in diagnostic procedures.
Please note that product labelling (such as kit insert, product label, and kit box) may be different compared to the company branding. Please contact your local representative for further details.