DNA and RNA from Blood
Workflow for nucleic acid extraction from a large volume of whole blood
The workflow for DNA and RNA purification from a large volume of blood allows the efficient isolation of high yields of ultra-pure intact nucleic acids from up to 12 samples of 10 ml blood in parallel. The large sample volume workflow is ideal for laboratories requiring very high yields of nucleic acids for their downstream applications. The workflow is based on chemagen's proprietary M-PVA Magnetic Bead technology and a dedicated configuration of the chemagic™ 360 instrument. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire procedure. The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification. The isolated high-quality DNA and RNA are suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage.
Kits for large volumes of blood
Product overview: DNA purification
| Catalog no. | Kit name | Instrument | Format | Preps/Kit | Input | Typical yield | Standard Elution Volume |
|---|---|---|---|---|---|---|---|
| CMG-715 | chemagic™ DNA Blood 7k Kit H12 | chemagic™ 360/MSM I | tube | 250 | up to 7 ml | 100 - 350 μg | 500 µl |
| CMG-704 | chemagic™ DNA Blood 10k Kit H12 | chemagic™ 360/MSM I | tube | 250 | up to 10 ml | 150 - 500 μg | 1.2 - 1.5 ml |
Genomic DNA isolation yields from different blood volumes with the chemagic™ MSM I.
0.5 ml blood (average value from 50 samples)
2 ml blood (average value from 2,000 samples)
5 ml blood (average value from 1,500 samples)
7 ml blood (average value from 1,200 samples)
10 ml blood (average value from 50 samples)
Typical DNA concentration obtained from a healthy donor is 20 - 40 μg per ml blood.
Instrument for the large-volume workflow from blood
Instrument for the processing of large volumes of blood
Workflow for nucleic acid purification from large-volume blood samples
- Automation: chemagic™ 360 with 12 Rod Head configuration
- Workflow capacity: from 12 up to 190 samples per day
- Sample volume: from 4 ml up to 10 ml
- Up to 300 μg of genomic DNA extracted from 10 ml of blood in a single run
- Compact design of the nucleic acid purification instrument
Contact us to discuss if this workflow fits your needs or to request a demo
For research use only. Not for use in diagnostic procedures.