microRNA isolation from plasma

Quality Automated microRNA Purification with Minimized Hands-On Time

The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.

With only 10 minutes of hands-on time within a 90-minute total processing time, the chemagic™ miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.

Sprungziel Features

Key Benefits:

  • Consistent and scalable small RNA extraction with comparable yields to manual methods.
  • Fast, automated workflow (10 min hands-on time) with on-board lysis and no heating required.
  • Suitable for plasma, serum or extracellular vesicles at input volumes of 200 µl or 1 ml.
  • Suitable for various downstream assays and proven compatibility with PerkinElmer NEXTFLEX® Small RNA-Seq Kit (refer App Note).
  • Full sample tracking with barcode reading and bidirectional LIMS communication capability.

 

Figure: microRNA was isolated from 0.2 ml (top) and 1.0 ml (bottom) plasma from three different donors with the chemagic kits on the chemagic 360 instrument and manually with competitor Q. Analysis by Qubit™ microRNA Assay Kit.

Sprungziel Customer Review

Customer Review

Customer Review

“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples, thus we look forward to use the kit for high throughput applications.”

Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine Finland FIMM, University of Helsinki

Sprungziel Product Overview

Product overview for Automated miRNA isolation

Catalog No.Kit NameInstrumentSamples/BatchPreps/KitSample VolumesProcessing Time
CMG-1224chemagic
miRNA 200 Kit H96
chemagic 360/MSM Iup to 969600.2 ml90 min
(incl. 10 min hands-on time)
CMG-1223chemagic
miRNA 1k Kit H24
chemagic 360/MSM Iup to 242401 ml90 min
(incl. 10 min hands-on time)

Sprungziel Manuals

Download Manual

Catalog No.Kit NamePDF Protocol
CMG-1224chemagic™ miRNA 200 Kit H96Protocol CMG-1224 chemagic miRNA 200 Kit H96
CMG-1223chemagic™ miRNA 1k Kit H24Protocol CMG-1223 chemagic miRNA 1k Kit H24

Sprungziel Application Notes

Application Notes

For research use only. Not for use in diagnostic procedures.