Workflow solutions for medium- to low-throughput nucleic acid isolation from buffy coats
The workflows for medium- to low-throughput DNA and RNA purification from buffy coats are designed to meet the needs of laboratories requiring the isolation of nucleic acids from tens to a hundred samples per day, with the possibility of easily scaling up to over 150 samples. The ability of handling volumes up to two milliliters per sample makes these workflows ideal for downstream applications requiring high yields of nucleic acids.
Product overview for medium-throughput workflows
| Catalog No. | Kit Name | Instrument | Input | Typical Yield | Format | Preps/Kit |
|---|---|---|---|---|---|---|
| CMG-1480 | chemagic™ Prime DNA Buffy Coat 500 Kit H24 | chemagic™ Prime 4/8 | 500 µl | up to 200 μg depending on the cell count | 24 well | 240 |
| CMG-1080 | chemagic™ DNA Buffy Coat 500 Kit H24 | chemagic™ 360/MSM I | 500 µl | up to 200 μg depending on the cell count | 24 well | 240 |
| CMG-1484 | chemagic™ Prime Total RNA Kit H24 | chemagic™ Prime 4/8 | 2.5 ml | Depending on sample quality | 24 well | 240 |
| CMG-1084 | chemagic™ Total RNA Kit H24 | chemagic™ 360/MSM I | 2.5 ml | Depending on sample quality | 24 well | 240 |
Product overview for low-throughput workflows
| Catalog No. | Kit Name | Instrument | Input | Typical Yield | Format | Preps/Kit |
|---|---|---|---|---|---|---|
| CMG-728 | chemagic™ DNA Buffy Coat 2k Kit H12 | chemagic™ 360/MSM I | up to 2 ml | up to 500 µg depending on the cell count | tube | 250 |
| CMG-1083 | chemagic™ Total RNA Kit H12 | chemagic™ 360/MSM I | up to 2.5 ml | Depending on sample quality | tube | 250 |
Based on the combination of dedicated instruments and chemagen proprietary M-PVA Magnetic Bead technology, the high-throughput DNA and RNA purification workflows ensure the efficient isolation of high yields of ultra-pure nucleic acids both from fresh buffy coats or from fractions that have been stored at 4°C. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire workflow.
Instruments for medium- to low-throughput workflows from buffy coats
Workflow for medium- and low-throughput nucleic acid purification from buffy coats
- Automation: chemagic™ 360 instrument with 12 Rod Head configuration
- Workflow capacity: from 12 up to 150 samples per day
- Sample volume: from 500 μl to 2 ml
- Optional integration with liquid handlers for primary sample transfer into plates compatible with the extraction procedure
- Compact design of the nucleic acid purification instrument
- The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification
- High-quality DNA and RNA suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage
Automated workflow for medium-throughput nucleic acid purification from buffy coat samples and optional PCR reaction setup
- Automation: chemagic Prime™ instrument with 12 Rod Head configuration
- Workflow capacity: from 12 up to 120 samples per day
- Sample volume: from 500 μl to 2 ml
- Automated transfer of primary samples to plates for nucleic acid extraction
- Up to 24 samples processed in a single run
- Optional automated PCR reaction setup after nucleic acid purification
- The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification
- High-quality DNA and RNA suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage
Contact us to discuss if this workflow fits your needs or to request a demo
We offer highly flexible financing options to allow the setup of the workflow you need in your lab in the most convenient terms. Please contact us for more information.
For research use only. Not for use in diagnostic procedures.