Kit Categories: Sample Type

chemagic DNA Soil kits

DNA isolation from plants

DNA purification from soil

The chemagic™ DNA Soil Kit is designed for the automated purification of total DNA from soil.

Soil DNA kits

Kit features

  • Proprietary technology based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Please find below the available kit for DNA isolation from soil using the chemagic 360 instrument.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-1054chemagic™
DNA Soil 250 Kit H96		chemagic™ 360/MSM Iup to 250 mg soildepending on the sample material96 well960

Application Note

For research use only. Not for use in diagnostic procedures.

DNA from Blood Spots

Kits for DNA purification from DBS

Kits for DNA Purification from Dried Blood Spots (DBS)

The chemagic™ DNA Blood kits for purification of DNA from DBS are designed for the processing of 3.0 – 3.5 mm punches or 13 mm punches on the chemagic™ 360 instrument.

DNA isolation from blood spots

Kit features

Please find below a selection of available kits for DNA isolation from dried blood spots using the chemagic™ 360 instrument.

chemagic DNA Blood Spot Kits for automation

Catalog No.Kit NameInstrumentSamples/BatchPreps/KitSample SizeRun Time
CMG-779chemagic
DNA Blood Spot 13 Kit H96		chemagic 360/MSM Iup to 969608 - 13 mm52 min
(excl. lysis)
CMG-1130chemagic
DNA Blood Spot 3 Kit H96		chemagic 360/MSM Iup to 969603 - 3.5 mm46 min
(excl. lysis)

For research use only. Not for use in diagnostic procedures.

High volume cfDNA purification

Kits for cfDNA purification from high volumes of plasma

The chemagic™ cfDNA kits for high volume cfDNA purification from plasma are designed for the processing of 6 - 10 ml, 15 ml or 18 ml plasma with minimal hands-on time on the chemagic™ 360 instrument . The kits are designed for use with human plasma samples derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

  • Designed for use with the chemagic™ 360 instrument for high-throughput cfDNA purification
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • 1 - 30 ng cfDNA/ml plasma

Please find below a selection of available kits for cfDNA isolation from high volumes of plasma (6 to 18 ml) using chemagic™ instruments, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentSamples/
Batch		Preps/
Kit		Sample VolumesYieldProcessing TimePurchase online
CMG-1310*chemagic
cfDNA 10k Kit H24		chemagic 360/MSM I242406-10 ml1 - 30 ng/ml plasma (qPCR)115 min
(incl. 20 min hands-on time)
CMG-1318chemagic
cfDNA 18k Kit H12		chemagic 360/MSM I12Up to 25010, 15 or 18 ml1 - 30 ng/ml plasma (qPCR)125 min
(incl. 15 min hands-on time)

*Additional accessories required: 3 quantity chemagic 13 mL Double Bottom Rack H24 (art. No. CMG-13005340)

Functional Principle

Functional Principle

The chemagic™ cfDNA for medium volumes of plasma are based on chemagic™ Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-1310chemagic™ cfDNA 10k Kit H24Protocol CMG-1310 chemagic cfDNA 10k Kit H24

Application Notes

For research use only. Not for use in diagnostic procedures.

miRNA purification

Kits for miRNA purification from plasma

Kits for microRNA (miRNA) purification from plasma

The chemagic™ miRNA kits for purification of circulating cell-free small RNA from plasma are designed for the processing of 0.2  or 1 ml plasma with minimal hands-on time on the chemagic™ 360 instrument. The kits are designed for the use with plasma, serum or extracellular vesicles.

Medium volume cfDNA purification from plasma

Kit features

  • Designed for the use with the chemagic™ 360 instrument for miRNA purification in 24-well or 96-well format
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • Consistent and scalable small RNA extraction with comparable yields to manual methods.
  • Suitable for various downstream assays and proven compatibility (refer App Note).

Please find below a selection of available kits for miRNA isolation from 0.2 or 1 ml plasma using  the chemagic™ 360 instrument.

chemagic miRNA Kits for automation

Catalog No.Kit NameInstrumentSamples/BatchPreps/KitSample VolumesProcessing Time
CMG-1224chemagic
miRNA 200 Kit H96		chemagic 360/MSM Iup to 969600.2 ml90 min
(incl. 10 min hands-on time)
CMG-1223chemagic
miRNA 1k Kit H24		chemagic 360/MSM Iup to 242401 ml90 min
(incl. 10 min hands-on time)

Download Manual

Catalog No.Kit NamePDF Protocol
CMG-1224chemagic™ miRNA 200 Kit H96Protocol CMG-1224 chemagic miRNA 200 Kit H96
CMG-1223chemagic™ miRNA 1k Kit H24Protocol CMG-1223 chemagic miRNA 1k Kit H24

Customer review

“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples, thus we look forward to use the kit for high throughput applications.”

Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine Finland FIMM, University of Helsinki

Moon SM, Kim JH, Kim SK, Kim S, Kwon HJ, Bae JS, Lee S, Lee HS, Choi MY, Jeon BH, Jeong BH, Lee K, Kim HK, Kim J, Um SW. Clinical Utility of Combined Circulating Tumor Cell and Circulating Tumor DNA Assays for Diagnosis of Primary Lung Cancer. Anticancer Res. 2020 Jun;40(6):3435-3444.

For research use only. Not for use in diagnostic procedures.

Low volume cfDNA purification

Kit for cfDNA purification from low volumes of plasma

The chemagic™ cfDNA 1.5k Kit H96 for low-volume cfDNA purification from plasma is designed for the processing of 0.5 - 1.5 ml plasma with minimal hands-on time on the chemagic 360 instrument. The kit  is designed for the use with plasma samples derived from EDTA, citrate or other collection tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

  • Designed for the use with the chemagic™ 360 instrument for high-throughput cfDNA purification
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • 1 - 30 ng cfDNA/ml plasma

Kit for chemagic™ automation

Catalog No.Kit NameFormatPreps/KitInputYieldProcessing Time
CMG-1396chemagic™ cfDNA 1.5k Kit H9696-well960500 µl - 1.5 ml1 - 30 ng/ml plasma (qPCR)100 min
(incl. 15 min hands-on time)

Functional Principle

Functional Principle

The chemagic cfDNA for medium volumes of plasma are based on chemagic Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic 360 excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-1396chemagic cfDNA 1.5k Kit H96Protocol CMG-1396 chemagic cfDNA 1.5k Kit H96

Application Notes

For research use only. Not for use in diagnostic procedures.

DNA kit for food samples

Kits for DNA purification from food

The chemagic™ Food Basic Kit is designed for manual DNA extraction and purification of genomic DNA from 200 mg food samples of mainly plant origin. Lysis of food material and binding conditions are optimized to obtain both high yield and high quality of the genomic DNA. The purified DNA is suitable for downstream applications such as PCR and GMO detection. To increase the sensitivity of detection for difficult samples such as processed food, it is possible to scale up the sample size using the chemagic Food Extension Kit to obtain lysates from 2 g of food samples material.

Food DNA kits

Kit features

  • Proprietary technology based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

For research use only. Not for use in diagnostic procedures.

Plant DNA kits

DNA isolation from plants

DNA purification from plant tissue

The chemagic™ DNA Plant Kits are designed for the automated or manual purification of total DNA from plant tissue.

Plant DNA kits

Kit features

  • Proprietary technology based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Please find below a selection of available kits for DNA isolation from plant tissue using chemagic™ instrument or liquid handling workstations. A manual kit solution is available on request.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-795chemagic™
DNA Plant 20 Kit H96		chemagic™ 360/MSM Iup to 20 mg plant tissuedepending on the sample material96 well960

Kits for liquid handling instruments

Product no.Kit nameInstrumentInputFormatPreps/kit
CMG-1029chemagic™ DNA Plant Kit LHMost liquid handling workstations20 mg96 well960

Application Note

For research use only. Not for use in diagnostic procedures.

Sequencing reaction clean-up kits

Sequencing reaction clean-up

Kits for sequencing reaction clean-up

The chemagic SEQ Pure Kit was developed to purify products from sequencing reactions such as BigDye® terminator reactions.

Sequencing reaction clean-up

Kit features

  • Optimized for the use with liquid handling workstations
  • Proprietary M-PVA Magnetic Beads for optimal PCR product yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Please find below a selection of available kits for sequencing reaction clean-up using liquid handling workstations.

Kits for liquid handling instruments

Product no.Kit nameInstrumentInputFormatPreps/kit
CMG-451chemagic™ SEQ Pure 10 Kit LHMost liquid handling workstations10 μl96 well960
CMG-458chemagic™ SEQ Pure 20 Kit LHMost liquid handling workstations20 µl96 well960

For research use only. Not for use in diagnostic procedures.

PCR purification kits

Kits for PCR product purification

The PCR Pure chemagic™ kits are designed for the efficient recovery of amplified DNA from PCR reactions and removal of residual primers.

PCR purification kits

Kit features

  • Optimized for the use with liquid handling workstations
  • Proprietary M-PVA Magnetic Beads for optimal PCR product yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Please find below a selection of available kits for the isolation of PCR reaction products using liquid handling workstations.

Kits for liquid handling instruments

Product no.Kit nameInstrumentInputFormatPreps/kit
CMG-451chemagic™ PCR Pure 10 Kit LHMost liquid handling workstations10 μl96 well960
CMG-450chemagic™ PCR Pure 20 Kit LHMost liquid handling workstations20 μl96 well960

For research use only. Not for use in diagnostic procedures.

Bacteria nucleic acid kits

Kits for nucleic acid purification from bacteria

The Bacteria chemagic™ kits are designed for the efficient isolation of high-quality nucleic acids from bacteria.

Bacteria nucleic acid kits

Kit features

Please find below a selection of available kits for nucleic acid isolation from bacteria using chemagic™ instruments.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-799chemagic™
DNA Bacteria Kit H96		chemagic™ 360/MSM I1 ml (pellet)depending on OD96 well960

For research use only. Not for use in diagnostic procedures.