cfDNA extraction technical note

cfDNA isolation

Automated Circulating Cell-Free DNA Purification with the chemagic™ 360 Instrument

The purification of cell-free DNA (cfDNA) is challenging because of the low concentrations, its instability and the small fragment sizes. Nevertheless the information it delivers is highly
valuable for cancer studies and research of prenatal testing using technologies as NGS and ddPCR. The chemagic™ cfDNA kits enable the automated and efficient extraction of cfDNA from 0.5 - 5 ml plasma in 24-well or 96-well format in maximal 2 hours with minimal hands-on time. The chemagic™ cfDNA kits show equivalent yields as well as purities compared to the manual silica based gold standard kit.

Efficient cfDNA extraction with the chemagic 360 instrument

Comparable yields of cfDNA from 1 - 5 ml plasma with chemagic cfDNA kits compared to competitor.

Plasma was prepared from fresh blood of a healthy donor by centrifugation at 2000 x g for 20 minutes. The supernatant was collected and centrifuged once more at 3300 x g for 30 minutes. The plasma was frozen at -20 °C and thawed at 30 °C prior to cfDNA extraction. cfDNA was extracted from 1, 2, and 5 ml plasma with the chemagic™ cfDNA kits on the chemagic™ 360 instrument (blue lanes) and manually with competitor (green lanes). The eluted DNA was loaded on the HT X-Mark LabChip running the HT DNA NGS 3K assay on the LabChip GX Touch HT Nucleic Acid Analyzer (LM: Lower Marker, UM: Upper Marker).

Features of the chemagic™ cfDNA Purification Workflows

PerkinElmer chemagen developed three chemagic™ cfDNA kits to isolate cfDNA from 0.5 - 5 ml fresh and frozen plasma derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes within 90 - 120 minutes with the chemagic™ 360 instrument . The chemagic™ cfDNA kits are based on chemagen technology using M-PVA Magnetic Beads for the isolation of cfDNA. The cfDNA binds to paramagnetic beads, which are magnetically separated from the sample material. During subsequent steps, contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument excludes cross-contamination and ensures safe handling of infectious sample material.

For more information

For more information, please download the technical note about automated cfDNA purification.

For research use only. Not for use in diagnostic procedures.