Optimal Recovery of Microbial DNA for 16S Sequencing from Murine Gastrointestinal Tract Tissue Through Bead Mill Homogenization and Automated Nucleic Acid Extraction

The microbiome is a vast interconnected network of viruses, fungi, and bacteria found inside and on the surface of all entities, both living and non-living¹. This network affects nearly all daily homeostatic functions in humans, including digestion, cognition, and immunity¹. To aid in the discovery of new relationships and to delve deeper into current relationships we as humans have with our resident microorganisms, researchers must use methods that are not only repeatable and scalable but also maximize the yields of target analytes from challenging matrices.

In the following application note,  we demonstrate the utility of OMNI Bead Ruptor Elite™ Bead Mill Homogenizer in combination with the chemagic™ 360 instrument for a reliable workflow that address the lysis and recovery of DNA from liquid culture samples and the recovery of total microbial DNA from solid tissue samples.

1. Amon, P., & Sanderson, I. (2017). What is the microbiome? Archives of Disease in Childhood - Education & Practice Edition, 102(5), 257–260. https://doi.org/10.1136/archdischild-2016-311643