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Automated nucleic acid extraction from liver tissue application note

Automated Nucleic Acid Isolation from Liver Tissue

Nucleic acid extraction from liver tissue: An integrated solution utilizing the Bead Ruptor Elite™ Bead Mill Homogenizer, chemagic™ 360 Nucleic Acid Extractor and JANUS® G3 Automated Workstation

Nucleic acid extraction from tissue commonly precedes downstream applications like quantitative PCR and Next Generation Sequencing, which are methods utilized to drive genomic-based research. The Bead Ruptor Elite™ Bead Mill Homogenizer and optimized 2 mL Bead Kits provide power and efficiency in sample homogenization releasing analytes of interest, while the chemagic™ 360 nucleic acid extractor automatically extracts genomic material from homogenized samples at low, medium and high throughput demands.

Here in, we show integration of the Bead Ruptor Elite™ Bead Mill Homogenizer with the chemagic™ 360 nucleic acid extractor and Janus® G3 automated workstation platforms by creating a workflow that incorporates sample preparation of tissue, custom-integrated liquid handling and automated nucleic acid extraction and provides the end user with nucleic acid suitable for further genomic analysis.

Automated nucleic acid extraction from liver

Automated nucleic acid isolation from liver tissue

Liver DNA concentration from 12 samples

Lysate from samples 1-12 was prepared with Pro-K and RNase A. Average A260 /A280 from samples 1-12 was 1.90.

Nucleic Acid Extraction and Analysis

After centrifugation step, 100 µl of tissue lysate was transferred to 12 separate wells of a 96-well plate using the JANUS G3 automated workstation and customized Reagent Liquid Transfer Winprep program. Similarly, 100 µl Lysis Buffer provided with the chemagic Tissue DNA Kit (CMG-723) was transferred to each well containing the lysate. Next, 6 µl of Proteinase-K, included in the chemagic Tissue DNA kit, was manually transferred to all wells along with 5 µl RNase A. The plate was then incubated at 37 °C for 10 minutes. During this time, Elution Buffer and Magnetic Beads were transferred into respective plates using a Reagent Liquid Transfer Protocol on the JANUS G3 automated workstation. After incubation, the DNA extraction was carried out using the chemagic 360 nucleic acid extractor:

For research use only. Not for use in diagnostic procedures.