Cost-Effective Multiplex Testing of Tick-Borne Pathogens using the HDPCR™ Tick-Borne Pathogen Research Use Only Panel with Automated chemagic 360™ Extraction Integration

Tick-borne diseases are the most common vector-borne diseases in the United States. Reported cases of tickborne disease infection have constantly been rising over the last fifteen years, partly caused by increased outdoor activities and suburban living preferences. Nucleic acid extraction with subsequent Real-time PCR are widely used for pathogen identification from extracted samples. However, the detection capacity for existing real-time PCR methods is limited by the number of fluorescent detection channels in the instrument. Therefore, it is necessary to increase multiplexing capability in each detection channel to increase detection efficiency in a single reaction.

Here, we present the HDPCR™ Tick-Borne Pathogen Research Use Only Panel with chemagic™ 360 system integration using whole blood with spiked-in tick-borne synthetic targets and extraction with chemagic™ 360 Viral DNA/gDNA 200 Kit H96 for workflow evaluation.