Automated RNA purification from blood stored in S-Monovette® RNA Exact from SARSTEDT with the chemagic™ 360 instrument
Studies of transcriptome from whole blood are often limited due to the instability of RNA caused by nucleases and temperature after sample collection, storage, and a suboptimal isolation. In this Application Note, we show the successful stabilization of RNA by the S-Monovette® RNA Exact tubes from SARSTEDT in combination with automated RNA isolation with the chemagic™ Total RNA 9k Kit H24 (CMG-1084-S) on the chemagic™ 360 instrument. High-quality RNA was extracted after up to seven days stored at room temperature and up to 14 days stored at 4 °C.
High-quality RNA after incubation at 22° C.
Excellent RNA integrity for five donors was obtained from isolated RNA stored in S-Monovette® RNA Exact tubes at 22 °C. High-quality RNA (RQS > 8) was isolated after two days storage at 22 °C and even good RNA quality (RQS > 6) was obtained for up to seven days storage.
Features of the chemagic™ Total RNA 9k Kit H24 Workflow
PerkinElmer chemagen developed the chemagic™ Total RNA 9k Kit H24 (CMG-1084-S) to isolate RNA from 2.4 ml fresh and frozen blood stabilized in the S-Monovette® RNA Exact tubes with the chemagic™ 360 instrument. The chemagic™ Total RNA 9k Kit H24 is based on the chemagen technology using M-PVA Magnetic Beads for the isolation of RNA. Nucleic acids bind to paramagnetic beads, which are magnetically separated from the sample material. During subsequent steps, contaminants are removed, DNA is digested, and the purified RNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument excludes cross-contamination and ensures safe handling of infectious sample material.
For more information
For more information, please download the technical note about automated RNA purification.
For research use only. Not for use in diagnostic procedures.