Automated RNA purification from PAXgene

Automated RNA purification from PAXgene Blood RNA Tubes with the chemagic 360 instrument

Automated RNA purification from PAXgene® Blood RNA Tubes with the chemagic™ 360 instrument

Transcriptome analysis from whole blood is an interesting segment of research, but also difficult. RNA quality and yields are influenced by nucleases, inappropriate temperatures, and de novo syntheses after sample collection and storage and have an immediate effect on gene expression analyses. The use of blood collection tubes simplifies this application enormously by stabilizing RNA during sample collection and storage. Here, we show the automated isolation of high-quality RNA with the chemagic™ Total RNA Kit H24 on the chemagic™ 360 instrument from whole blood stored in PAXgene® Blood RNA Tubes. Samples were stored for several days at room temperature and 4 °C, and high-quality RNA was obtained without any inhibition in RT-PCR assays.

Automated RNA purification from Paxgene

Automated RNA purification from Paxgene

Reliable RNA quality after storage at 4° C.

Appropriate RNA integrity for five donors obtained from PAXgene® RNA Tubes after storage at 4 °C. Good RNA integrity could be obtained from samples stored at 4°C up to 14 days.

Features of the chemagic™ Total RNA Kit H24 Workflow

PerkinElmer chemagen developed the chemagic™ Total RNA Kit H24 to isolate RNA from 2.5 ml fresh and frozen blood stabilized in the PAXgene® Blood RNA Tube with the chemagic™ 360 instrument. The chemagic™ Total RNA Kit H24 kit is based on chemagen technology using M-PVA Magnetic Beads for the isolation of RNA. The RNA binds to paramagnetic beads, which are magnetically separated from the sample material. During subsequent steps, contaminants are removed, DNA is digested, and the purified RNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument excludes cross contamination and ensures safe handling of infectious sample material.

For more information

For more information, please download the technical note about automated RNA purification from PAXgene® Blood RNA Tubes.

For research use only. Not for use in diagnostic procedures.