DNA and RNA from Blood
Workflow solutions for high-throughput nucleic acid isolation from whole blood
The workflows for the high-throughput purification of DNA and RNA from blood are ideal for laboratories routinely requiring high-quality nucleic acid isolation from large numbers of samples. Depending on the needs of your lab, Revvity solutions can include the automated transfer of primary samples to plates compatible with the nucleic acid extraction unit and the option of automated PCR setup to streamline sample analysis. Based on the combination of dedicated instruments and the proprietary M-PVA Magnetic Bead-based technology, Revvity's workflows for high-throughput DNA and RNA purification from blood ensure the efficient isolation of high yields of ultra-pure intact nucleic acids from 50 µl up to 500 µl whole blood. The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire procedure. The purified DNA and RNA are suitable for virtually all molecular analysis methods (NGS, MLPA, qPCR, etc.) and long-term storage.
Kits for high-throughput DNA purification
Product overview: DNA purification
| Catalog No. | Kit Name | Instrument | Format | Preps/Kit | Input | Typical yield | Standard Elution Volume |
|---|---|---|---|---|---|---|---|
| CMG-717 | chemagic™ DNA Blood 200 Kit H96 | chemagic™ 360/MSM I | 96 well | 960 | up to 200 μl | 4 - 8 μg | 100 µl |
| CMG-1091 | chemagic™ DNA Blood 400 Kit H96 | chemagic™ 360/MSM I | 96 well | 960 | up to 400 μl | 12 - 16 μg | 250 µl |
| CMG-1491 | chemagic™ Prime DNA Blood 400 Kit H96 | chemagic™ Prime 4/8 | 96 well | 960 | up to 400 μl | 12 - 16 μg | 250 µl |
| CMG-1534 | Junior gDNA Kit | chemagic™ Prime Junior | 48 well | 480 | up to 500 μl | 4 - 16 μg | 100 µl |
Kits for high-throughput RNA purification
Product overview: RNA purification
| Catalog No. | Kit Name | Instrument | Format | Preps/Kit | Input | Standard Elution Volume |
|---|---|---|---|---|---|---|
| CMG-1212 | chemagic™ RNA Blood Kit H96 | chemagic™ 360/MSM I | 96 well | 960 | 1.25 ml blood or 10 mg tissue | 100 µl |
| CMG-1412 | chemagic™ Prime RNA Blood Kit H96 | chemagic™ Prime 4/8 | 96 well | 960 | 1.25 ml blood or 10 mg tissue | 100 µl |
Instruments for high-throughput workflows from blood
Instruments for high-throughput workflows from blood
Workflow for high- and very high-throughputs
- Automation: chemagic™ 360 instrument with 96 Rod Head configuration
- Workflow capacity: from 96 up to 4000 samples per day
- Compact instrument design
- Optional integration with liquid handlers for primary sample transfer compatible with the extraction procedure (e.g., JANUS® G3 Blood iQ™ workstation, or explorer™ G3 workstations)
- Ultra-fast protocols available for nucleic acid extraction in less than 30 minutes
Automated workflow for very high-throughput and optional PCR reaction setup
- Automation: chemagic™ Prime instrument with 96 Rod Head configuration
- Workflow capacity: from 96 up to 1000 samples per day
- Automated transfer of primary samples to plates for nucleic acid extraction
- Up to 192 samples processed in a single run
- Further automated applications possible like PCR reaction setup after nucleic acid purification, pipetting in storage systems, or aliquoting operations
Application Note
Application Note
For research use only. Not for use in diagnostic procedures.