Medium volume saliva DNA kits

Kits for DNA purification from medium volumes of saliva

The medium volume chemagic™ Saliva kits for genomic DNA purification are designed for the efficient isolation of high-quality DNA from 500 μl to 4 ml of saliva.

Medium volume saliva DNA kits

Medium volume saliva DNA kit features

Kit features

  • Optimized for the use with chemagic™ instruments for high- and medium-throughput nucleic acid isolation
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Flexible input volume from 500 μl to 5 ml of saliva
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Please find below a selection of available kits for DNA isolation from medium volumes of saliva (500 μl to 4 ml) using chemagic™ instruments, liquid handling workstations, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-1481chemagic™ Prime
DNA Saliva 4k Kit H24
chemagic™ Prime 4/8up to 4 mlup to 40 µg depending on the input quality24 well240
CMG-1081chemagic™
DNA Saliva 4k Kit H24
chemagic™ 360/MSM Iup to 4 mlup to 40 µg depending on the input quality24 well240
CMG-1092chemagic™
DNA Saliva 2k Kit H12
chemagic™ 360/MSM Iup to 2 mlup to 30 μg depending on the sample materialtube250
CMG-1035chemagic™
DNA Saliva 4k Kit H12
chemagic™ 360/MSM Iup to 4 mlup to 80 μg depending on the sample materialtube250

For research use only. Not for use in diagnostic procedures.

Low volume saliva DNA kits

Kits for DNA purification from low volumes of saliva

The low volume Saliva chemagic™ kits for genomic DNA purification are designed for the efficient isolation of high quality DNA up to 600 μl of saliva.

Low volume saliva DNA kits

Low volume saliva DNA kit features

Kit features

Please find below a selection of available kits for DNA isolation from low volumes of saliva (up to 600 μl) using chemagic™ instruments. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-1437chemagic™ Prime
DNA Saliva 600 Kit H96
chemagic™ Prime 4/8600 μlup to 10 µg depending on the input quality96 well960
CMG-1037chemagic™
DNA Saliva 600 Kit H96
chemagic™ 360/MSM I600 μlup to 10 µg depending on the input quality96 well960

For research use only. Not for use in diagnostic procedures.

Large volume buffy coat DNA kits

Kits for DNA purification from large volumes of buffy coat fractions

The large volume buffy coat chemagic™ kits for genomic DNA purification are designed for the isolation of very high yields of high quality DNA from 5 to 10 ml of buffy coat fraction.

Large volume buffy coat DNA kits

Large volume DNA purification from buffy coat: kit features

Kit features

Please find below a selection of available kits for DNA isolation from large volumes of buffy coat fraction (5 to 10 ml) using chemagic™ instruments, liquid handling workstations, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kit for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-728chemagic™
DNA Buffy Coat 2k Kit H12
chemagic™ 360/MSM Iup to 2 mlup to 500 µg depending on the cell counttube250

For research use only. Not for use in diagnostic procedures.

Medium volume buffy coat DNA kits

Kits for DNA purification from medium volumes of buffy coat fractions

The medium volume Buffy Coat chemagic™ kits for genomic DNA purification are designed for the isolation of very high yields of high quality DNA from up to 500 μl buffy coat fraction.

DNA isolation from buffy coats

Kit features

  • Optimized for the use with chemagic™ instruments for nucleic acid isolation
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Flexible input volume from up to  500 μl
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Please find below a selection of available kits for DNA isolation from medium volumes of buffy coat fraction (up to 500 μl) using chemagic™ instruments. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-1480chemagic™ Prime
DNA Buffy Coat 500 Kit H24
chemagic™ Prime 4/8500 µlup to 200 μg depending on the cell count24 well240
CMG-1080chemagic™
DNA Buffy Coat 500 Kit H24
chemagic™ 360/MSM I500 µlup to 200 μg depending on the cell count24 well240

For research use only. Not for use in diagnostic procedures.

Low volume buffy coat DNA kits

Kits for DNA purification from low volumes of buffy coat fractions

The low volume chemagic™ Buffy Coat kits for genomic DNA purification are designed for the isolation of very high yields of high-quality DNA from up to 200 μl buffy coat fraction.

Low volume buffy coat DNA kits

Low volume DNA purification from buffy coats: kit features

Kit features

Please find below a selection of available kits for DNA isolation from low volumes of buffy coat fraction (up to 200 µl) using chemagic™ instruments. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputTypical yieldFormatPreps/kit
CMG-1413chemagic™ Prime
DNA Buffy Coat 200 Kit H96
chemagic™ Prime 4/8200 μlup to 60 µg depending on the cell count96 well960
CMG-713chemagic™
DNA Buffy Coat 200 Kit H96
chemagic™ 360/MSM I200 μlup to 60 µg depending on the cell count96 well960

For research use only. Not for use in diagnostic procedures.

Large volume pathogen nucleic acid kits

Large volume pathogen nucleic acids kits

Kits for pathogen nucleic acid purification from large volumes of plasma or serum

The Large-volume Viral DNA/RNA chemagic™ kits are designed for the efficient isolation of high-quality DNA and RNA from 4 to 10 ml of plasma or serum.

Large volume pathogen nucleic acid kits

Large volume pathogen nucleic acid kit features

Kit features

For research use only. Not for use in diagnostic procedures.

Medium volume pathogen nucleic acid kits

Medium volume pathogen nucleic acid kits

Kits for pathogen nucleic acid purification from medium volumes of plasma or serum

The chemagic™ Body Fluid kits are designed for the efficient isolation of high-quality DNA and RNA from 1 ml of plasma or serum.

Medium volume pathogen nucleic acid kits

Medium volume pathogen nucleic acid kit features

Kit features

Medium volume pathogen nucleic acid kits

Please find below a selection of available kits for pathogen nucleic acid isolation from medium volumes of plasma or serum (up to 1 ml) using chemagic™ instruments. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputFormatPreps/kit
CMG-1406chemagic™ Prime
Body Fluid 1k Kit H24
chemagic™ Prime 4/81 ml24 well240
CMG-1103chemagic™
Body Fluid 1k Kit H24
chemagic™ 360/MSM I2 ml24 well240

For research use only. Not for use in diagnostic procedures.

Low volume pathogen nucleic acid kits

Low volume pathogen nucleic acid kits

Kits for pathogen nucleic acid purification from low volumes of plasma and serum

The Low-volume Viral DNA/RNA chemagic™ kits are designed for the efficient high-throughput isolation of high-quality DNA and RNA from 50 μl to 1 ml of plasma or serum.

Low volume pathogen nucleic acid kits

Low volume pathogen nucleic acid kit features

Kit features

  • Optimized for the use with automated nucleic acid isolation instruments for high-throughput nucleic acid extraction
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • Typical sensitivity: 6.7 I.U./ml plasma pool (HBV)

Please find below a selection of available kits for viral DNA/RNA isolation from low volumes of plasma or serum (50 µl to 1 ml) using chemagic™ instruments, liquid handling workstations, or manual handling. For different sample volumes please refer to the kit selection page.

Low volume pathogen nucleic acid kits

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentInputFormatPreps/Kit
CMG-1449chemagic™ Prime
Viral NA/gDNA 200 Kit H96
chemagic™ Prime 4/8up to 200 µl96 well960
CMG-1431chemagic™ Prime
Body Fluid 1k Kit H96
chemagic™ Prime 4/8up to 1000 µl96 well960
CMG-1049chemagic™
Viral NA/gDNA 200 Kit H96
chemagic™ 360/MSM Iup to 200 µl96 well960
CMG-1033chemagic™
Viral DNA/RNA 300 Kit H96
chemagic™ 360/MSM Iup to 300 µl96 well960
CMG-1131chemagic™
Body Fluid 1k Kit H96
chemagic™ 360/MSM Iup to 1000 µl96 well960
CMG-2021Prepito®
NA Body Fluid Kit
chemagic Prepito®up to 200 µl12 well180
CMG-2017Prepito®
Viral DNA/RNA300 Kit
chemagic Prepito®up to 300 µl12 well180
CMG-2018Prepito®
Viral DNA/RNA1k Kit
chemagic Prepito®up to 1000 µl12 well180
CMG-1521Junior
Pathogen NA Kit
chemagic™ Prime Juniorup to 1000 µl48 well480

Kit for liquid handling instruments

Catalog No.Kit NameInstrumentInputFormatPreps/Kit
CMG-1201chemagic™ Viral DNA/RNA Kit LHMost liquid handling workstations200 µl96 well960

Kits for manual nucleic extraction

Kits for manual nucleic extraction

Catalog no.Kit nameInputNo. of preps
CMG-192chemagic™ Viral NA/gDNA Kit200 µl50
CMG-1033-P*chemagic™ Viral DNA/RNA Kit300 µl960
*This kit solution is also suitable for liquid handling instruments and other magnetic bead based nucleic acid purification instruments.

For research use only. Not for use in diagnostic procedures.

Medium volume cfDNA purification

Kits for cfDNA purification from medium volumes of plasma

The chemagic™ cfDNA kits for medium volume cfDNA purification from plasma are designed for the processing of 2 - 5 ml plasma with minimal hands-on time on the chemagic™ 360 instrument, the chemagic™ Prime™ instrument, or manual processing. The kits are designed for the use with human plasma samples derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

Please find below a selection of available kits for cfDNA isolation from medium volumes of plasma (1 to 5 ml) using chemagic™ instruments, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentFormatPreps/KitInputYieldProcessing Time
CMG-1444chemagic™
Prime cfDNA 5k Kit H24
chemagic™
Prime 4/8
24 well2403 - 5 ml1 - 30 ng/ml plasma (qPCR)240 min
CMG-1304chemagic™
cfDNA 5k Kit H24
chemagic™
360 /MSM I
24 well2403 - 5 ml1 - 30 ng/ml plasma (qPCR)100 min
(incl. 15 min hands-on time)
CMG-1302chemagic™
cfDNA 2k Kit H24
chemagic™
360 /MSM I
24 well2401 - 2 ml1 - 30 ng/ml plasma (qPCR)90 min
(incl. 15 min hands-on time)

Kit for manual nucleic extraction

Product No.Kit NameAccessories required
Preps / Kit
Sample Volumes Processing Time
CMG-134chemagic™ cfDNA 5k Kitchemagic™ Stand 2 x 12
chemagic Magnetic Stand Type F
chemagic cfDNA Stand 12 (Optional)
1 - 5 ml60-90 min (depending on user experience)

Functional Principle

Functional Principle

The chemagic™ cfDNA for medium volumes of plasma are based on chemagic™ Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument or the chemagic™ Prime™ instrument excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-134chemagic™ cfDNA 5k Kit (manual)Protocol CMG-134 chemagic cfDNA 5k Kit
CMG-1304chemagic™ cfDNA 5k Kit H24Protocol CMG-1304 chemagic cfDNA 5k Kit H24
CMG-1302chemagic™ cfDNA 2k Kit H24Protocol CMG-1302 chemagic cfDNA 2k Kit H24

Application Notes

For research use only. Not for use in diagnostic procedures.

Medium volume viral nucleic acids and genomic DNA Kits

Kits for viral nucleic acid and genomic DNA purification from medium volumes of body fluids

The medium volume Viral DNA/RNA gDNA chemagic™ kits are designed for the efficient high-throughput isolation of high quality DNA and RNA from up to 1 ml of body fluids. The particular benefit of these kits is the ability to isolate nucleic acids from different kinds of body fluids such as blood, serum, plasma, urine, stool suspensions, nasal-, vaginal- and buccal swabs, sputum, cerebrospinal fluid, puncture, cell suspensions, amniotic fluid, and others. The kit can be used to purify both viral nucleic acids and genomic DNA for research use only.

viral nucleic acids and genomic DNA Kits

Medium volume viral nucleic acids and genomic DNA Kits

Kit features

  • Designed for the use with automated nucleic acid isolation instruments for high-throughput nucleic acid extraction
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Ideal for isolation of pathogen DNA from blood and blood-contaminated samples
  • Efficient isolation of genomic DNA from body fluids
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature

Medium volume viral nucleic acids and genomic DNA Kits

Please find below a selection of available kits for viral nucleic acid and genomic DNA isolation from medium volumes of body fluids (up to 1 ml) using chemagic™ instruments. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentInputFormatPreps/Kit
CMG-1406chemagic™ Prime Body Fluid 1k Kit H24chemagic™ Prime 4/8up to 1 ml24 well240
CMG-1103chemagic™ Body Fluid 1k Kit H24chemagic™ 360/MSM Iup to 1 ml24 well240

For research use only. Not for use in diagnostic procedures.