High-throughput DNA and RNA purification from buffy coats

Workflow solutions for high-throughput nucleic acid isolation from buffy coats

The PerkinElmer workflows for high-throughput nucleic acid purification are ideal for laboratories that need to extract DNA and RNA from large numbers of buffy coats. Depending on the needs of your lab, PerkinElmer solutions can include the automated transfer of the isolated buffy coat fractions to plates compatible with the nucleic acid extraction unit and automated PCR setup to streamline the analysis.

Product overview for DNA isolation from buffy coats

Catalog No.Kit NameInstrumentInputTypical YieldFormatPreps/Kit
CMG-1413chemagic™ Prime
DNA Buffy Coat 200 Kit H96
chemagic™ Prime 4/8200 μlup to 60 µg depending on the cell count96 well960
CMG-713chemagic™
DNA Buffy Coat 200 Kit H96
chemagic™ 360/MSM I200 μlup to 60 µg depending on the cell count96 well960

Product overview for RNA isolation from buffy coats

Catalog No.Kit NameInstrumentFormatPreps/KitInputStandard Elution
Volume
CMG-1212chemagic™ RNA Blood Kit H96chemagic™ 360/MSM I96 well9601.25 ml blood or 10 mg tissue100 µl
CMG-1412chemagic™ Prime RNA Blood Kit H96chemagic™ Prime 4/896 well9601.25 ml blood or 10 mg tissue100 µl

Based on the combination of dedicated instruments and chemagen's proprietary M-PVA Magnetic Bead-based technology, PerkinElmer high-throughput DNA and RNA purification workflows ensure the efficient isolation of high yields of ultra-pure nucleic acids both from fresh buffy coats or from fractions that have been stored at 4°C. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire workflow.

Instruments for high-throughput workflows from buffy coats

Instruments for high-throughput workflows from buffy coats

Workflow for high-throughput nucleic acid purification from buffy coats

  • Automation: chemagic™ 360 instrument with 96 Rod Head configuration
  • Workflow capacity: from 96 up to 1000 samples per day
  • Sample volume: from 100 to 500 μl
  • Compact instrument design to save lab space
  • Optional integration with liquid handlers for primary sample transfer into plates compatible with the extraction procedure
  • The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification
  • High-quality DNA and RNA suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage

Automated workflow for very high-throughput nucleic acid purification from buffy coat fractions and optional PCR reaction setup

  • Automation: chemagic™ Prime instrument with 96 Rod Head configuration
  • Sample volume: from 100 to 500 μl
  • Automated transfer of the isolated buffy coat fractions from storage tubes to plates for nucleic acid extraction
  • Up to 192 samples processed in a single run
  • Optional automated PCR reaction setup after nucleic acid purification
  • The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification
  • High-quality DNA and RNA suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage

Contact us to discuss if this workflow fits your needs or to request a demo.

PerkinElmer offers highly flexible financing options to allow the setup of the workflow you need in your lab in the most convenient terms. Please contact us for more information.

For research use only. Not for use in diagnostic procedures.