High-throughput DNA and RNA from blood

DNA and RNA from Blood

Workflow solutions for high-throughput nucleic acid isolation from whole blood

The workflows for the high-throughput purification of DNA and RNA from blood are ideal for laboratories routinely requiring high-quality nucleic acid isolation from large numbers of samples. Depending on the needs of your lab, Revvity solutions can include the automated transfer of primary samples to plates compatible with the nucleic acid extraction unit and the option of automated PCR setup to streamline sample analysis. Based on the combination of dedicated instruments and the proprietary M-PVA Magnetic Bead-based technology, Revvity's workflows for high-throughput DNA and RNA purification from blood ensure the efficient isolation of high yields of ultra-pure intact nucleic acids from 50 µl up to 500 µl whole blood. The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire procedure. The purified DNA and RNA are suitable for virtually all molecular analysis methods (NGS, MLPA, qPCR, etc.) and long-term storage.

Kits for high-throughput DNA purification

Product overview: DNA purification

Catalog No.Kit NameInstrumentFormatPreps/KitInputTypical yieldStandard Elution
Volume
CMG-717chemagic™ DNA Blood 200 Kit H96chemagic™ 360/MSM I96 well960up to 200 μl4 - 8 μg100 µl
CMG-1091chemagic™ DNA Blood 400 Kit H96chemagic™ 360/MSM I96 well960up to 400 μl12 - 16 μg250 µl
CMG-1491chemagic™ Prime DNA Blood 400 Kit H96chemagic™ Prime 4/896 well960up to 400 μl12 - 16 μg250 µl
CMG-1534Junior gDNA Kitchemagic™ Prime Junior48 well480up to 500 μl4 - 16 μg100 µl

Kits for high-throughput RNA purification

Product overview: RNA purification

Catalog No.Kit NameInstrumentFormatPreps/KitInputStandard Elution
Volume
CMG-1212chemagic™ RNA Blood Kit H96chemagic™ 360/MSM I96 well9601.25 ml blood or 10 mg tissue100 µl
CMG-1412chemagic™ Prime RNA Blood Kit H96chemagic™ Prime 4/896 well9601.25 ml blood or 10 mg tissue100 µl

Instruments for high-throughput workflows from blood

Instruments for high-throughput workflows from blood

Workflow for high- and very high-throughputs

  • Automation: chemagic™ 360 instrument with 96 Rod Head configuration
  • Workflow capacity: from 96 up to 4000 samples per day
  • Compact instrument design
  • Optional integration with liquid handlers for primary sample transfer compatible with the extraction procedure (e.g., JANUS® G3 Blood iQ workstation, or explorer™ G3 workstations)
  • Ultra-fast protocols available for nucleic acid extraction in less than 30 minutes

Automated workflow for very high-throughput and optional PCR reaction setup

  • Automation: chemagic™ Prime instrument with 96 Rod Head configuration
  • Workflow capacity: from 96 up to 1000 samples per day
  • Automated transfer of primary samples to plates for nucleic acid extraction
  • Up to 192 samples processed in a single run
  • Further automated applications possible like PCR reaction setup after nucleic acid purification, pipetting in storage systems, or aliquoting operations

Application Note

Application Note

For research use only. Not for use in diagnostic procedures.