Kits for cfDNA purification from medium volumes of plasma
The chemagic™ cfDNA kits for medium volume cfDNA purification from plasma are designed for the processing of 2 - 5 ml plasma with minimal hands-on time on the chemagic™ 360 instrument, the chemagic™ Prime™ instrument, or manual processing. The kits are designed for the use with human plasma samples derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes. In addition, fresh and frozen plasma can be used.
Kit features
- Designed for the use with the chemagic™ 360 instrument or chemagic™ Prime™ instrument for medium-throughput cfDNA purification
- Proprietary technology based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
- Disposable plasticware included
- Convenient packaging to reduce reagent waste
- Reagents can be stored at room temperature
- 1 - 30 ng cfDNA/ml plasma
Please find below a selection of available kits for cfDNA isolation from medium volumes of plasma (1 to 5 ml) using chemagic™ instruments, or a manual procedure. For different sample volumes please refer to the kit selection page.
Kits for chemagic™ automation
| Catalog No. | Kit Name | Instrument | Format | Preps/Kit | Input | Yield | Processing Time |
|---|---|---|---|---|---|---|---|
| CMG-1444 | chemagic™ Prime cfDNA 5k Kit H24 | chemagic™ Prime 4/8 | 24 well | 240 | 3 - 5 ml | 1 - 30 ng/ml plasma (qPCR) | 240 min |
| CMG-1304 | chemagic™ cfDNA 5k Kit H24 | chemagic™ 360 /MSM I | 24 well | 240 | 3 - 5 ml | 1 - 30 ng/ml plasma (qPCR) | 100 min (incl. 15 min hands-on time) |
| CMG-1302 | chemagic™ cfDNA 2k Kit H24 | chemagic™ 360 /MSM I | 24 well | 240 | 1 - 2 ml | 1 - 30 ng/ml plasma (qPCR) | 90 min (incl. 15 min hands-on time) |
Kit for manual nucleic extraction
| Product No. | Kit Name | Accessories required Preps / Kit | Sample Volumes | Processing Time |
|---|---|---|---|---|
| CMG-134 | chemagic™ cfDNA 5k Kit | chemagic™ Stand 2 x 12 chemagic Magnetic Stand Type F chemagic cfDNA Stand 12 (Optional) | 1 - 5 ml | 60-90 min (depending on user experience) |
Functional Principle
Functional Principle
The chemagic™ cfDNA for medium volumes of plasma are based on chemagic™ Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument or the chemagic™ Prime™ instrument excludes cross contamination and ensures safe handling of infectious sample material.
Plasma Preparation Protocol
Plasma Preparation Protocol
It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.
- Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
- Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
- Centrifuge the plasma sample at 3300 x g for 30 minutes.
- Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
- For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.
Download Manual
| Catalog No. | Kit name | PDF protocol |
|---|---|---|
| CMG-134 | chemagic™ cfDNA 5k Kit (manual) | Protocol CMG-134 chemagic cfDNA 5k Kit |
| CMG-1304 | chemagic™ cfDNA 5k Kit H24 | Protocol CMG-1304 chemagic cfDNA 5k Kit H24 |
| CMG-1302 | chemagic™ cfDNA 2k Kit H24 | Protocol CMG-1302 chemagic cfDNA 2k Kit H24 |
Reference
Application Notes
For research use only. Not for use in diagnostic procedures.