DNA and RNA from Blood
Workflows for medium-throughput nucleic acid isolation from whole blood
The chemagic workflows for medium-throughput automated DNA and RNA purification from blood are designed to meet the needs of laboratories who need to isolate nucleic acids from 10 to 100 samples per day, with the possibility of easy upscaling to 250 samples. The ability to handle volumes up to four milliliters per sample in a single run makes these workflows ideal for downstream applications requiring high yields of nucleic acids (e.g. multiple testing). Based on proprietary M-PVA Magnetic Bead technology and instruments, the medium-throughput workflows for DNA and RNA purification ensure the efficient isolation of high yields of ultra-pure intact nucleic acids. The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire procedure. The isolated high-quality DNA and RNA are suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage.
Kits for medium-throughput DNA purification
Product overview: DNA purification
| Catalog. No. | Kit Name | Instrument | Format | Preps/Kit | Input | Typical Yield | Standard Elution Volume |
|---|---|---|---|---|---|---|---|
| CMG-1097 | chemagic™ DNA Blood 2k Kit H24 | chemagic™ 360/MSM I | 24 well | 240 | up to 2 ml | 30 - 100 μg | 300 - 500 µl |
| CMG-1497 | chemagic™ Prime DNA Blood 2k Kit H24 | chemagic™ Prime 4/8 | 24 well | 240 | up to 2 ml | 30 - 100 μg | 300 - 500 µl |
| CMG-1074 | chemagic™ DNA Blood 4k Kit H24 | chemagic™ 360/MSM I | 24 well | 240 | up to 4 ml | 60 - 200 μg | 300 - 500 µl |
| CMG-1474 | chemagic™ Prime DNA Blood 4k Kit H24 | chemagic™ Prime 4/8 | 24 well | 240 | up to 4 ml | 60 - 200 μg | 300 - 500 µl |
| CMG-1534 | Junior gDNA Kit | chemagic™ Prime Junior | 48 well | 480 | up to 500 µl | 4 - 16 μg | 100 µl |
Application data
Genomic DNA isolation from blood using the chemagic™ 360 instrument
Genomic DNA from initial sample volume of 4 ml of blood and has been eluted in 500 μl. The average yield is 63 μg per ml of blood, with a DNA concentration of 363 ng/μl ± 163. The typical DNA quality as measured by A260/A280 is 1,98 ± 0,26.
Kits for medium-throughput RNA purification
Product overview: RNA purification
| Catalog No. | Kit Name | Instrument | Format | Preps/Kit | Input | Typical Yield | Standard Elution Volume |
|---|---|---|---|---|---|---|---|
| CMG-1084 | chemagic™ Total RNA Kit H24 | chemagic™ 360/MSM I | 24 well | 240 | 2.5 ml | 60 - 200 μg | 100 - 200 µl |
| CMG-1484 | chemagic™ Prime Total RNA Kit H24 | chemagic™ Prime 4/8 | 24 well | 240 | 2.5 ml | 30 - 80 μg | 100 - 200 µl |
Instruments for medium-throughput workflows from blood
Instruments for medium-throughput workflows from blood
A compact automated solution
- Automation: chemagic™ 360 instrument with 24 Rod Head configuration
- Optional integration with liquid handlers for primary sample transfer compatible with the extraction procedure (e.g., JANUS® G3 Blood iQ™ workstation or explorer™ G3 workstations)
- Ultra-fast protocols available for nucleic acid extraction in less than 45 minutes
- Compact design of the nucleic acid purification instrument
Automated solution with liquid handling unit
- Automation: chemagic™ Prime instrument with 24 Rod Head configuration
- Automated transfer of primary samples to plates for nucleic acid extraction
- Up to 48 samples processed in a single run
- Further automated applications possible like PCR reaction setup after nucleic acid purification, aliquoting operations, or pipetting in storage systems
Contact us to discuss if one of these workflows fit your needs or to request a demo.
Automated solution with liquid handling unit
- Automation: chemagic™ Prime Junior instrument
- Automated transfer of primary samples to plates for nucleic acid extraction
- Up to 48 samples processed in a single run
- Further automated applications possible like PCR reaction setup after nucleic acid purification, aliquoting operations, or pipetting in storage systems
Contact us to discuss if one of these workflows fit your needs or to request a demo.
Application Note
Application Notes
For research use only. Not for use in diagnostic procedures.