Medium volume cfDNA purification

Kits for cfDNA purification from medium volumes of plasma

The chemagic™ cfDNA kits for medium volume cfDNA purification from plasma are designed for the processing of 2 - 5 ml plasma with minimal hands-on time on the chemagic™ 360 instrument, the chemagic™ Prime™ instrument, or manual processing. The kits are designed for the use with human plasma samples derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

Please find below a selection of available kits for cfDNA isolation from medium volumes of plasma (1 to 5 ml) using chemagic™ instruments, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentFormatPreps/KitInputYieldProcessing Time
Prime cfDNA 5k Kit H24
Prime 4/8
24 well2403 - 5 ml1 - 30 ng/ml plasma (qPCR)240 min
cfDNA 5k Kit H24
360 /MSM I
24 well2403 - 5 ml1 - 30 ng/ml plasma (qPCR)100 min
(incl. 15 min hands-on time)
cfDNA 2k Kit H24
360 /MSM I
24 well2401 - 2 ml1 - 30 ng/ml plasma (qPCR)90 min
(incl. 15 min hands-on time)

Kit for manual nucleic extraction

Catalog No.Kit NameAccessoriesInputTypical YieldPreps/Kit
CMG-134chemagic™ cfDNA 5k Kitchemagic™ Stand 2 x 12
chemagic™ Stand 50k Type B
1 - 5 mldepending on the sample material40

Functional Principle

Functional Principle

The chemagic™ cfDNA for medium volumes of plasma are based on chemagic™ Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument or the chemagic™ Prime™ instrument excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-134chemagic™ cfDNA 5k Kit (manual)Protocol CMG-134 chemagic cfDNA 5k Kit
CMG-1304chemagic™ cfDNA 5k Kit H24Protocol CMG-1304 chemagic cfDNA 5k Kit H24
CMG-1302chemagic™ cfDNA 2k Kit H24Protocol CMG-1302 chemagic cfDNA 2k Kit H24

For research use only. Not for use in diagnostic procedures.