Kit Categories: Plasma and Serum

High volume cfDNA purification

Kits for cfDNA purification from high volumes of plasma

The chemagic™ cfDNA kits for high volume cfDNA purification from plasma are designed for the processing of 6 - 10 ml, 15 ml or 18 ml plasma with minimal hands-on time on the chemagic™ 360 instrument . The kits are designed for use with human plasma samples derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

  • Designed for use with the chemagic™ 360 instrument for high-throughput cfDNA purification
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • 1 - 30 ng cfDNA/ml plasma

Please find below a selection of available kits for cfDNA isolation from high volumes of plasma (6 to 18 ml) using chemagic™ instruments, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentSamples/
Batch		Preps/
Kit		Sample VolumesYieldProcessing TimePurchase online
CMG-1310*chemagic
cfDNA 10k Kit H24		chemagic 360/MSM I242406-10 ml1 - 30 ng/ml plasma (qPCR)115 min
(incl. 20 min hands-on time)
CMG-1318chemagic
cfDNA 18k Kit H12		chemagic 360/MSM I12Up to 25010, 15 or 18 ml1 - 30 ng/ml plasma (qPCR)125 min
(incl. 15 min hands-on time)

*Additional accessories required: 3 quantity chemagic 13 mL Double Bottom Rack H24 (art. No. CMG-13005340)

Functional Principle

Functional Principle

The chemagic™ cfDNA for medium volumes of plasma are based on chemagic™ Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-1310chemagic™ cfDNA 10k Kit H24Protocol CMG-1310 chemagic cfDNA 10k Kit H24

Application Notes

For research use only. Not for use in diagnostic procedures.

miRNA purification

Kits for miRNA purification from plasma

Kits for microRNA (miRNA) purification from plasma

The chemagic™ miRNA kits for purification of circulating cell-free small RNA from plasma are designed for the processing of 0.2  or 1 ml plasma with minimal hands-on time on the chemagic™ 360 instrument. The kits are designed for the use with plasma, serum or extracellular vesicles.

Medium volume cfDNA purification from plasma

Kit features

  • Designed for the use with the chemagic™ 360 instrument for miRNA purification in 24-well or 96-well format
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • Consistent and scalable small RNA extraction with comparable yields to manual methods.
  • Suitable for various downstream assays and proven compatibility (refer App Note).

Please find below a selection of available kits for miRNA isolation from 0.2 or 1 ml plasma using  the chemagic™ 360 instrument.

chemagic miRNA Kits for automation

Catalog No.Kit NameInstrumentSamples/BatchPreps/KitSample VolumesProcessing Time
CMG-1224chemagic
miRNA 200 Kit H96		chemagic 360/MSM Iup to 969600.2 ml90 min
(incl. 10 min hands-on time)
CMG-1223chemagic
miRNA 1k Kit H24		chemagic 360/MSM Iup to 242401 ml90 min
(incl. 10 min hands-on time)

Download Manual

Catalog No.Kit NamePDF Protocol
CMG-1224chemagic™ miRNA 200 Kit H96Protocol CMG-1224 chemagic miRNA 200 Kit H96
CMG-1223chemagic™ miRNA 1k Kit H24Protocol CMG-1223 chemagic miRNA 1k Kit H24

Customer review

“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples, thus we look forward to use the kit for high throughput applications.”

Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine Finland FIMM, University of Helsinki

Moon SM, Kim JH, Kim SK, Kim S, Kwon HJ, Bae JS, Lee S, Lee HS, Choi MY, Jeon BH, Jeong BH, Lee K, Kim HK, Kim J, Um SW. Clinical Utility of Combined Circulating Tumor Cell and Circulating Tumor DNA Assays for Diagnosis of Primary Lung Cancer. Anticancer Res. 2020 Jun;40(6):3435-3444.

For research use only. Not for use in diagnostic procedures.

Low volume cfDNA purification

Kit for cfDNA purification from low volumes of plasma

The chemagic™ cfDNA 1.5k Kit H96 for low-volume cfDNA purification from plasma is designed for the processing of 0.5 - 1.5 ml plasma with minimal hands-on time on the chemagic 360 instrument. The kit  is designed for the use with plasma samples derived from EDTA, citrate or other collection tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

  • Designed for the use with the chemagic™ 360 instrument for high-throughput cfDNA purification
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • 1 - 30 ng cfDNA/ml plasma

Kit for chemagic™ automation

Catalog No.Kit NameFormatPreps/KitInputYieldProcessing Time
CMG-1396chemagic™ cfDNA 1.5k Kit H9696-well960500 µl - 1.5 ml1 - 30 ng/ml plasma (qPCR)100 min
(incl. 15 min hands-on time)

Functional Principle

Functional Principle

The chemagic cfDNA for medium volumes of plasma are based on chemagic Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic 360 excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-1396chemagic cfDNA 1.5k Kit H96Protocol CMG-1396 chemagic cfDNA 1.5k Kit H96

Application Notes

For research use only. Not for use in diagnostic procedures.

Large volume pathogen nucleic acid kits

Large volume pathogen nucleic acids kits

Kits for pathogen nucleic acid purification from large volumes of plasma or serum

The Large-volume Viral DNA/RNA chemagic™ kits are designed for the efficient isolation of high-quality DNA and RNA from 4 to 10 ml of plasma or serum.

Large volume pathogen nucleic acid kits

Large volume pathogen nucleic acid kit features

Kit features

For research use only. Not for use in diagnostic procedures.

Medium volume pathogen nucleic acid kits

Medium volume pathogen nucleic acid kits

Kits for pathogen nucleic acid purification from medium volumes of plasma or serum

The chemagic™ Body Fluid kits are designed for the efficient isolation of high-quality DNA and RNA from 1 ml of plasma or serum.

Medium volume pathogen nucleic acid kits

Medium volume pathogen nucleic acid kit features

Kit features

Medium volume pathogen nucleic acid kits

Please find below a selection of available kits for pathogen nucleic acid isolation from medium volumes of plasma or serum (up to 1 ml) using chemagic™ instruments. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Product no.Kit nameInstrumentInputFormatPreps/kit
CMG-1406chemagic™ Prime
Body Fluid 1k Kit H24		chemagic™ Prime 4/81 ml24 well240
CMG-1103chemagic™
Body Fluid 1k Kit H24		chemagic™ 360/MSM I2 ml24 well240

For research use only. Not for use in diagnostic procedures.

Low volume pathogen nucleic acid kits

Low volume pathogen nucleic acid kits

Kits for pathogen nucleic acid purification from low volumes of plasma and serum

The Low-volume Viral DNA/RNA chemagic™ kits are designed for the efficient high-throughput isolation of high-quality DNA and RNA from 50 μl to 1 ml of plasma or serum.

Low volume pathogen nucleic acid kits

Low volume pathogen nucleic acid kit features

Kit features

  • Optimized for the use with automated nucleic acid isolation instruments for high-throughput nucleic acid extraction
  • Proprietary technology  based on the use of M-PVA Magnetic Beads for optimal nucleic acid yield and purity
  • Disposable plasticware included
  • Convenient packaging to reduce reagent waste
  • Reagents can be stored at room temperature
  • Typical sensitivity: 6.7 I.U./ml plasma pool (HBV)

Please find below a selection of available kits for viral DNA/RNA isolation from low volumes of plasma or serum (50 µl to 1 ml) using chemagic™ instruments, liquid handling workstations, or manual handling. For different sample volumes please refer to the kit selection page.

Low volume pathogen nucleic acid kits

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentInputFormatPreps/Kit
CMG-1449chemagic™ Prime
Viral NA/gDNA 200 Kit H96		chemagic™ Prime 4/8up to 200 µl96 well960
CMG-1431chemagic™ Prime
Body Fluid 1k Kit H96		chemagic™ Prime 4/8up to 1000 µl96 well960
CMG-1049chemagic™
Viral NA/gDNA 200 Kit H96		chemagic™ 360/MSM Iup to 200 µl96 well960
CMG-1033chemagic™
Viral DNA/RNA 300 Kit H96		chemagic™ 360/MSM Iup to 300 µl96 well960
CMG-1131chemagic™
Body Fluid 1k Kit H96		chemagic™ 360/MSM Iup to 1000 µl96 well960
CMG-2021Prepito®
NA Body Fluid Kit		chemagic Prepito®up to 200 µl12 well180
CMG-2017Prepito®
Viral DNA/RNA300 Kit		chemagic Prepito®up to 300 µl12 well180
CMG-2018Prepito®
Viral DNA/RNA1k Kit		chemagic Prepito®up to 1000 µl12 well180
CMG-1521Junior
Pathogen NA Kit		chemagic™ Prime Juniorup to 1000 µl48 well480

Kit for liquid handling instruments

Catalog No.Kit NameInstrumentInputFormatPreps/Kit
CMG-1201chemagic™ Viral DNA/RNA Kit LHMost liquid handling workstations200 µl96 well960

Kits for manual nucleic extraction

Kits for manual nucleic extraction

Catalog no.Kit nameInputNo. of preps
CMG-192chemagic™ Viral NA/gDNA Kit200 µl50
CMG-1033-P*chemagic™ Viral DNA/RNA Kit300 µl960
*This kit solution is also suitable for liquid handling instruments and other magnetic bead based nucleic acid purification instruments.

For research use only. Not for use in diagnostic procedures.

Medium volume cfDNA purification

Kits for cfDNA purification from medium volumes of plasma

The chemagic™ cfDNA kits for medium volume cfDNA purification from plasma are designed for the processing of 2 - 5 ml plasma with minimal hands-on time on the chemagic™ 360 instrument, the chemagic™ Prime™ instrument, or manual processing. The kits are designed for the use with human plasma samples derived from EDTA, citrate or Streck® Cellfree DNA BCT® tubes. In addition, fresh and frozen plasma can be used.

Medium volume cfDNA purification from plasma

Kit features

Please find below a selection of available kits for cfDNA isolation from medium volumes of plasma (1 to 5 ml) using chemagic™ instruments, or a manual procedure. For different sample volumes please refer to the kit selection page.

Kits for chemagic™ automation

Catalog No.Kit NameInstrumentFormatPreps/KitInputYieldProcessing Time
CMG-1444chemagic™
Prime cfDNA 5k Kit H24		chemagic™
Prime 4/8		24 well2403 - 5 ml1 - 30 ng/ml plasma (qPCR)240 min
CMG-1304chemagic™
cfDNA 5k Kit H24		chemagic™
360 /MSM I		24 well2403 - 5 ml1 - 30 ng/ml plasma (qPCR)100 min
(incl. 15 min hands-on time)
CMG-1302chemagic™
cfDNA 2k Kit H24		chemagic™
360 /MSM I		24 well2401 - 2 ml1 - 30 ng/ml plasma (qPCR)90 min
(incl. 15 min hands-on time)

Kit for manual nucleic extraction

Product No.Kit NameAccessories required
Preps / Kit		Sample Volumes Processing Time
CMG-134chemagic™ cfDNA 5k Kitchemagic™ Stand 2 x 12
chemagic Magnetic Stand Type F
chemagic cfDNA Stand 12 (Optional)		1 - 5 ml60-90 min (depending on user experience)

Functional Principle

Functional Principle

The chemagic™ cfDNA for medium volumes of plasma are based on chemagic™ Technology using M-PVA Magnetic Beads. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic™ 360 instrument or the chemagic™ Prime™ instrument excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
Please refer to the tube manufacturers specifications (max. centrifugation speed) for more information.
From 10 mL of whole blood approximately 4 - 5 mL plasma can be expected.

  1. Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  2. Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  3. Centrifuge the plasma sample at 3300 x g for 30 minutes.
  4. Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  5. For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

Download Manual

Catalog No.Kit namePDF protocol
CMG-134chemagic™ cfDNA 5k Kit (manual)Protocol CMG-134 chemagic cfDNA 5k Kit
CMG-1304chemagic™ cfDNA 5k Kit H24Protocol CMG-1304 chemagic cfDNA 5k Kit H24
CMG-1302chemagic™ cfDNA 2k Kit H24Protocol CMG-1302 chemagic cfDNA 2k Kit H24

Application Notes

For research use only. Not for use in diagnostic procedures.