Medium-throughput DNA/RNA from blood

DNA and RNA from Blood

Workflows for medium-throughput nucleic acid isolation from whole blood

The PerkinElmer chemagen workflows for medium-throughput automated DNA and RNA purification from blood are designed to meet the needs of laboratories who need to isolate nucleic acids from 10 to 100 samples per day, with the possibility of easy upscaling to 250 samples. The ability to handle volumes up to four milliliters per sample in a single run makes these workflows ideal for downstream applications requiring high yields of nucleic acids (e.g. multiple testing). Based on chemagen proprietary M-PVA Magnetic Bead technology and instruments, PerkinElmer medium-throughput workflows for DNA and RNA purification ensure the efficient isolation of high yields of ultra-pure intact nucleic acids. The chemagic™ Kits include all required reagents and disposable plasticware for nucleic acid purification. Sample barcoding and software compatibility with all major LIMSs ensure full sample traceability throughout the entire procedure. The isolated high-quality DNA and RNA are suitable for molecular analysis (NGS, MLPA, qPCR, etc.) and long-term storage.

Kits for medium-throughput DNA purification

Product overview: DNA purification

Catalog. No.Kit NameInstrumentFormatPreps/KitInputTypical YieldStandard Elution
Volume
CMG-1097chemagic™ DNA
Blood 2k Kit special H24
chemagic™
360/MSM I
24 well240up to 2 ml30 - 100 μg300 - 500 µl
CMG-1497chemagic™ Prime
DNA Blood 2k Kit H24
chemagic™
Prime 4/8
24 well240up to 2 ml30 - 100 μg300 - 500 µl
CMG-1074chemagic™ DNA
Blood 4k Kit special H24
chemagic™
360/MSM I
24 well240up to 4 ml60 - 200 μg300 - 500 µl
CMG-1474chemagic™ Prime
DNA Blood 4k Kit H24
chemagic™
Prime 4/8
24 well240up to 4 ml60 - 200 μg300 - 500 µl
Application data for medium-throughput DNA purification from blood

Application data

Genomic DNA isolation from blood using the chemagic™ 360 instrument

Genomic DNA from initial sample volume of 4 ml of blood and has been eluted in 500 μl. The average yield is 63 μg per ml of blood, with a DNA concentration of 363 ng/μl ± 163. The typical DNA quality as measured by A260/A280 is 1,98 ± 0,26.

Kits for medium-throughput RNA purification

Product overview: RNA purification

Catalog No.Kit NameInstrumentFormatPreps/KitInputTypical YieldStandard Elution
Volume
CMG-1084chemagic™
Total RNA Kit H24
chemagic™
360/MSM I
24 well2402.5 ml60 - 200 μg100 - 200 µl
CMG-1484chemagic™ Prime
Total RNA Kit special H24
chemagic™
Prime 4/8
24 well2402.5 ml30 - 80 μg100 - 200 µl

Instruments for medium-throughput workflows from blood

Instruments for medium-throughput workflows from blood

A compact automated solution

Automated solution with liquid handling unit

  • Automation: chemagic™ Prime instrument with 24 Rod Head configuration
  • Automated transfer of primary samples to plates for nucleic acid extraction
  • Up to 48 samples processed in a single run
  • Further automated applications possible like PCR reaction setup after nucleic acid purification, aliquoting operations, or pipetting in storage systems

Contact us to discuss if one of these workflows fit your needs or to request a demo.

Application Note

Application Notes

For research use only. Not for use in diagnostic procedures.